TOXICITY and RETINAL PENETRATION of INFLIXIMAB in PRIMATES

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dc.contributor.authorMelo, Gustavo B. [UNIFESP]
dc.contributor.authorMoraes Filho, Milton N. [UNIFESP]
dc.contributor.authorRodrigues, Eduardo B. [UNIFESP]
dc.contributor.authorRegatieri, Caio V. [UNIFESP]
dc.contributor.authorDreyfuss, Juliana L. [UNIFESP]
dc.contributor.authorPenha, Fernando M. [UNIFESP]
dc.contributor.authorPinheiro, Marcelo M. [UNIFESP]
dc.contributor.authorCoimbra, Rita C. S. G. [UNIFESP]
dc.contributor.authorHaapalainen, Edna Freymuller [UNIFESP]
dc.contributor.authorFarah, Michel E. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:26:57Z
dc.date.available2016-01-24T14:26:57Z
dc.date.issued2012-03-01
dc.description.abstractPurpose: To evaluate the retinal penetration and toxicity of two doses of intravitreal infliximab in primates.Methods: Ten marmosets (Callithrix jacchus) were given intravitreal injection of 100 mu g or 400 mu g of infliximab, and balanced salt solution served as control. At baseline and after 24 hours (5 animals) and 7 days (the other 5), the eyes were examined by electroretinography. They were then killed (at 24 hours and 7 days) and assessed by light microscopy and transmission electron microscopy for toxicity and immunohistochemistry, using a biotinylated anti-human immunoglobulin G, to evaluate retinal penetration.Results: There was no difference over 50% of the electroretinography b-wave between baseline and the time points studied in all animals. Light and electron microscopy, and electroretinography analysis, showed no signs of toxicity in any of the animals. Strong presence of infliximab was observed in all retinal layers 7 days after intravitreal injection at both doses (100 and 400 mu g).Conclusion: Infliximab at doses of 100 and 400 mu g seemed to cause no damage to the retina 24 hours and 7 days after its intravitreal injection, and deeply penetrated all its layers, in primates. These results encourage future perspectives for the treatment of chronic inflammatory diseases of the retina in humans. RETINA 32: 606-612, 2012en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Mol Biol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Rheumatol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Electron Microscopy Ctr, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Mol Biol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Rheumatol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Electron Microscopy Ctr, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipPan-American Ophthalmological Foundation
dc.format.extent606-612
dc.identifierhttp://dx.doi.org/10.1097/IAE.0b013e3182252a23
dc.identifier.citationRetina-the Journal of Retinal and Vitreous Diseases. Philadelphia: Lippincott Williams & Wilkins, v. 32, n. 3, p. 606-612, 2012.
dc.identifier.doi10.1097/IAE.0b013e3182252a23
dc.identifier.issn0275-004X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34690
dc.identifier.wosWOS:000300907200025
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofRetina-the Journal of Retinal and Vitreous Diseases
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectelectron microscopyen
dc.subjectelectroretinographyen
dc.subjectinfliximaben
dc.subjectretinal penetrationen
dc.subjecttoxicityen
dc.titleTOXICITY and RETINAL PENETRATION of INFLIXIMAB in PRIMATESen
dc.typeinfo:eu-repo/semantics/article
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