Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model

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dc.contributor.authorArmstrong, Dinani Matoso Fialho de Oliveira
dc.contributor.authorArmstrong, Anderson da Costa
dc.contributor.authorFigueiredo, Regina Célia Bressan Queiroz
dc.contributor.authorFlorentino, Joao Eduardo
dc.contributor.authorSaad, Paulo Fernandes [UNIFESP]
dc.contributor.authorFox-Talbot, Karen
dc.contributor.authorHalushka, Marc Kenneth
dc.contributor.authorBerkowitz, Dan E.
dc.contributor.authorTaha, Murched Omar [UNIFESP]
dc.contributor.authorFagundes, Djalma José [UNIFESP]
dc.contributor.institutionSao Francisco Valley Federal University Department of Surgery
dc.contributor.institutionUNIVASF Department of Cardiology
dc.contributor.institutionResearch Center Aggeu Magalhaes Department of Microbiology
dc.contributor.institutionPernambuco Federal University
dc.contributor.institutionUNIVASF Department of Surgery
dc.contributor.institutionJohns Hopkins University Department of Pathology
dc.contributor.institutionJohns Hopkins University Department of Anesthesiology and Critical Care Medicine
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:45:21Z
dc.date.available2015-06-14T13:45:21Z
dc.date.issued2013-04-01
dc.description.abstractPURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.en
dc.description.affiliationSao Francisco Valley Federal University Department of Surgery
dc.description.affiliationUNIVASF Department of Cardiology
dc.description.affiliationResearch Center Aggeu Magalhaes Department of Microbiology
dc.description.affiliationPernambuco Federal University
dc.description.affiliationUNIVASF Department of Surgery
dc.description.affiliationJohns Hopkins University Department of Pathology
dc.description.affiliationJohns Hopkins University Department of Anesthesiology and Critical Care Medicine
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Surgery Operative Technique and Experimental Surgery Division
dc.description.affiliationUnifespUNIFESP, Department of Surgery Operative Technique and Experimental Surgery Division
dc.description.sourceSciELO
dc.format.extent282-287
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502013000400008
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 4, p. 282-287, 2013.
dc.identifier.doi10.1590/S0102-86502013000400008
dc.identifier.fileS0102-86502013000400008.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502013000400008
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/7683
dc.identifier.wosWOS:000317522300008
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectReperfusion Injuryen
dc.subjectMuscle, Skeletalen
dc.subjectPhosphodiesteraseen
dc.subjectInhibitorsen
dc.subjectCaspase 3en
dc.subjectRatsen
dc.titleSildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat modelen
dc.typeinfo:eu-repo/semantics/article
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