Protective effects of the exopolysaccharide Lasiodiplodan against DNA damage and inflammation induced by doxorubicin in rats: Cytogenetic and gene expression assays

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dc.citation.volume376
dc.contributor.authorMello, Michela Bianchi de
dc.contributor.authorMachado, Carla da Silva
dc.contributor.authorRibeiro, Diego Luis
dc.contributor.authorAissa, Alexandre Ferro
dc.contributor.authorBurim, Regislaine Valéria
dc.contributor.authorCunha, Mário Antonio Alves da
dc.contributor.authorBarcelos, Gustavo Rafael Mazzaron [UNIFESP]
dc.contributor.authorAntunes, Lusânia Maria Greggi
dc.contributor.authorBianchi, Maria Lourdes Pires
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageClare
dc.date.accessioned2020-07-17T14:03:11Z
dc.date.available2020-07-17T14:03:11Z
dc.date.issued2017
dc.description.abstractThe lasiodiplodan (LS) is a β-(1 → 6)-d-glucan produced by the fungus Lasiodiplodia theobromae and some of the biological activities of LS were reported as hypoglycemic, anticoagulant, anti-proliferative and anticancer action; however, its effects on DNA instability and modulation of gene expression are still unclear. Aims of study were investigate the genotoxic effects of lasiodiplodan, and its protective activity against DNA damage induced by doxorubicin (DXR) and its impact on the expression of genes associated with DNA damage and inflammatory response pathways. Therefore, Wistar rats were treated (15 days) orally with LS (5.0; 10 and 20 mg/kg bw) alone and in combination with DXR (15 mg/kg bw; administrated intraperitoneally on 14th day) as well as their respective controls: distilled water and DXR. Monitoring of DNA damage was assessed by comet and micronucleus (MN) assays and gene expression was evaluated by PCR-Arrays. Treatments with LS alone did not induce disturbances on DNA; when LS was given in combination with DXR, comet and MN formations were reduced to those found in the respective controls. Moreover, LS was able to reduce the disturbances on gene expressions induced by DXR treatment, since the animals that receive LS associated with DXR showed no alteration in the expression of genes related to DNA damage response. Also, DXR induced several up- and down-regulation of several genes associated to inflammatory process, while the animals that received LS + DXR had their gene expression patterns similar to those found in the control group. In conclusion, our results showed that LS did not induce disturbances on DNA stability and significantly reduce the DNA damage and inflammation caused by DXR exposure. In addition, we give further information concerning the molecular mechanisms associated to LS protective effects which seems to be a promising nutraceutical with chemopreventive potential.en
dc.description.affiliationUniv Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Genet, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Med Ribeirao Preto, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Tecnol Fed Parana, Dept Chem, Via Conhecimento,Km 01, BR-85503390 Pato Branco, Parana, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Hlth & Soc, Dept Biosci, Rua Silva Jardim 136, BR-11015020 Santos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Hlth & Soc, Dept Biosci, Rua Silva Jardim 136, BR-11015020 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipIDCNPq: 479233/2013-6pt
dc.description.sponsorshipIDFINEP: 01.09.0447.00pt
dc.format.extent66-74
dc.identifierhttps://dx.doi.org/10.1016/j.tox.2016.05.010
dc.identifier.citationToxicology. Clare, v. 376, p. 66-74, 2017.
dc.identifier.doi10.1016/j.tox.2016.05.010
dc.identifier.issn0300-483X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55210
dc.identifier.wosWOS:000393526700009
dc.language.isoeng
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofToxicology
dc.relation.ispartofIUTOX 9th Congress of Toxicology in Developing Countries (CTDC) / 19th Brazilian Congress of Toxicology (CBTox)
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAntigenotoxic effectsen
dc.subjectDNA-damageen
dc.subjectGene expressionen
dc.subjectInflammationen
dc.subjectLasiodiplodanen
dc.subjectNutraceuticalsen
dc.titleProtective effects of the exopolysaccharide Lasiodiplodan against DNA damage and inflammation induced by doxorubicin in rats: Cytogenetic and gene expression assaysen
dc.typeinfo:eu-repo/semantics/article
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