Evidence for neuronal release of isotopically labelled glycine from the rat ventral medullary surface in vivo

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dc.contributor.authordeOliveira, CVR
dc.contributor.authorAssumpção, Jurandyr D'Avila [UNIFESP]
dc.contributor.authorConfessor, Yara Queiroga [UNIFESP]
dc.contributor.authorGuertzenstein, Pedro Gaspar [UNIFESP]
dc.contributor.authorCravo, Sergio Luiz [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T16:23:59Z
dc.date.available2018-06-15T16:23:59Z
dc.date.issued1996-04-01
dc.description.abstractSpontaneous and stimulus-induced release of isotopically labelled glycine was studied in the superfused rat dorsal or ventral medullary surface in vivo. Superfusion of the ventral medullary surface of anesthetized (urethane, 1.2 g/kg, ip) male adult Wistar rats (250-350 g) with high K+ (40 mM) surrogate cerebrospinal fluid (CSF) produced an average increase of 45% over the spontaneous efflux of exogenously applied glycine (N = 5, P<0.01). In experiments in which the calcium of the CSF was replaced by an equimolar amount of magnesium, the increase in glycine efflux in response to high K+ was reduced to 15%, a value not statistically different from that observed in control experiments (N = 6). Veratridine stimulation evoked a large (80%) increase in glycine efflux (N = 5, P<0.001), which was inhibited by tetrodotoxin. High potassium or veratridine failed to modify spontaneous release of glycine on the dorsal medullary surface. Results obtained in control experiments showed that neither high K+ nor veratridine is effective in modifying spontaneous efflux of [H-3]-leucine or [H-3]-inulin on the ventral or dorsal medullary surface. These data support the hypothesis that glycine is a neurotransmitter on the ventral medullary surface and that it may be part of neural pathways involved in cardiorespiratory regulation present in this region.en
dc.description.affiliationUNIV FED SAO PAULO,ESCOLA PAULISTA MED,DEPT FISIOL,BR-04023900 SAO PAULO,SP,BRAZIL
dc.description.affiliationUnifespUNIV FED SAO PAULO,ESCOLA PAULISTA MED,DEPT FISIOL,BR-04023900 SAO PAULO,SP,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent527-532
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 29, n. 4, p. 527-532, 1996.
dc.identifier.issn0100-879X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43108
dc.identifier.wosWOS:A1996UE87000018
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectventral medullaen
dc.subjectglycineen
dc.subjectneurotransmitteren
dc.subjectamino aciden
dc.subjectveratridineen
dc.titleEvidence for neuronal release of isotopically labelled glycine from the rat ventral medullary surface in vivoen
dc.typeinfo:eu-repo/semantics/article
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