Toll-like receptor pathway signaling is differently regulated in neutrophils and peripheral mononuclear cells of patients with sepsis, severe sepsis, and septic shock
dc.contributor.author | Salomao, Reinaldo [UNIFESP] | |
dc.contributor.author | Brunialti, Milena K. C. [UNIFESP] | |
dc.contributor.author | Gomes, Natalia E. [UNIFESP] | |
dc.contributor.author | Mendes, Marialice E. [UNIFESP] | |
dc.contributor.author | Diaz, Ricardo Sobhie [UNIFESP] | |
dc.contributor.author | Komninakis, Shirley [UNIFESP] | |
dc.contributor.author | Machado, Flavia R. [UNIFESP] | |
dc.contributor.author | Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP] | |
dc.contributor.author | Rigato, Otelo [UNIFESP] | |
dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
dc.date.accessioned | 2016-01-24T13:52:07Z | |
dc.date.available | 2016-01-24T13:52:07Z | |
dc.date.issued | 2009-01-01 | |
dc.description.abstract | Objectives: Up- and down-regulation of inflammatory response was described in blood cells from septic patients, according to the stage of sepsis and the cells evaluated. This study aimed to evaluate the Toll-like receptor (TLR) signaling pathway gene expression in peripheral blood mononuclear cells (PBMC) and neutrophils in patients throughout the different stages of sepsis.Design: Prospective, observational study.Settings. Two emergency rooms and two intensive care units in one university and one teaching hospital.Patients and Controls., A total of 15 septic patients, five with sepsis, five with severe sepsis, and five with septic shock, in addition to five healthy volunteers were enrolled.Interventions: None.Measurements and Main Results: the Human-TLR Signaling Pathway, which comprises 84 genes related to TLR-mediated signal transduction, was evaluated by real time polymerase chain reaction in PBMC and neutrophils obtained from patients and controls. the fold change for each gene (2((-Delta Delta Ct))) was compared between the groups. Genes with fold changes greater than 2 and significant changes in Delta CT are reported as differently expressed. the told change ratios in PBMC gene expression between septic patients and healthy controls revealed a dynamic process according to the stage of sepsis, tending toward down-regulation of the TLR signaling pathway in PBMC in the more severe forms of the disease. However, the differential gene expression was restricted to five down-regulated genes in septic shock patients, which are found in the effector and downstream pathways. Neutrophils showed a different pattern of adaptation. Patients with sepsis, severe sepsis, and septic shock presented a broad gene upregulation, which included all functional groups evaluated and persisted throughout the stages of the disease.Conclusions: TLR-signaling pathway genes are differently regulated in PBMC and neutrophils of septic patients, and are dynamically modulated throughout the different stages of sepsis. (Grit Care Med 2009; 37:132-139) | en |
dc.description.affiliation | Universidade Federal de São Paulo, UNIFESP, Escola Paulista Med, Div Infect Dis, São Paulo, Brazil | |
dc.description.affiliation | Universidade Federal de São Paulo, UNIFESP, Escola Paulista Med, Dept Intens Care, São Paulo, Brazil | |
dc.description.affiliation | Universidade Federal de São Paulo, UNIFESP, Escola Paulista Med, Dept Gynecol, São Paulo, Brazil | |
dc.description.affiliationUnifesp | Universidade Federal de São Paulo, UNIFESP, Escola Paulista Med, Div Infect Dis, São Paulo, Brazil | |
dc.description.affiliationUnifesp | Universidade Federal de São Paulo, UNIFESP, Escola Paulista Med, Dept Intens Care, São Paulo, Brazil | |
dc.description.affiliationUnifesp | Universidade Federal de São Paulo, UNIFESP, Escola Paulista Med, Dept Gynecol, São Paulo, Brazil | |
dc.description.source | Web of Science | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorshipID | FAPESP: 04/15548-2 | |
dc.description.sponsorshipID | CNPq: 305101/2002-1 | |
dc.description.sponsorshipID | CNPq: 479666/2004-0 | |
dc.format.extent | 132-139 | |
dc.identifier | http://dx.doi.org/10.1097/CCM.0b013e318192fbaf | |
dc.identifier.citation | Critical Care Medicine. Philadelphia: Lippincott Williams & Wilkins, v. 37, n. 1, p. 132-139, 2009. | |
dc.identifier.doi | 10.1097/CCM.0b013e318192fbaf | |
dc.identifier.issn | 0090-3493 | |
dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/31202 | |
dc.identifier.wos | WOS:000262269900018 | |
dc.language.iso | eng | |
dc.publisher | Lippincott Williams & Wilkins | |
dc.relation.ispartof | Critical Care Medicine | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | Toll-like receptors | en |
dc.subject | intracellular signaling | en |
dc.subject | monocytes | en |
dc.subject | neutrophils | en |
dc.subject | sepsis | en |
dc.subject | septic shock | en |
dc.title | Toll-like receptor pathway signaling is differently regulated in neutrophils and peripheral mononuclear cells of patients with sepsis, severe sepsis, and septic shock | en |
dc.type | info:eu-repo/semantics/article |