Reactivity of antibodies from patients with acute and chronic paracoccidioidomycosis to a high molecular mass antigen from Paracoccidioides brasiliensis

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dc.contributor.authorMarquez, A. S.
dc.contributor.authorVicentini, A. P.
dc.contributor.authorOno, M. A.
dc.contributor.authorWatanabe, MAE
dc.contributor.authorCamargo, Z. P. de
dc.contributor.authorItano, E. N.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionAdolfo Lutz Inst
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:33Z
dc.date.available2016-01-24T12:37:33Z
dc.date.issued2005-01-01
dc.description.abstractYeast forms of Paracoccidioides brasiliensis produce polydispersed high molecular mass (h-MM) antigens. We investigated the antibodies to an h-MM antigen from P. brasiliensis by immunoblotting and ELISA in sera from paracoccidioidomycosis (PCM) patients. IgG from the sera of chronic PCIVI patients was able to recognize the h-MM antigen at a higher frequency in the cell-free antigen (CFA) (8/13) than in the somatic antigen (SA) (2/13), as assessed by immunoblotting. the CFA was fractionated by Sephadex G-200 chromatography, and fraction 17 (F17) with the h-MM antigen of approximately 366 kDa was used in ELISA to analyze specific levels of IgG and IgE. Patients with the chronic form showed significantly higher levels of IgG (P < 0.05) but not IgE (P > 0.05) to F17 by ELISA, compared to patients with the acute form or to healthy donors. in conclusion, CFA is better than SA as a source of the P. brasiliensis h-MM antigen. This study reveals a new characteristic to differentiate between the acute and chronic forms of PCM, by demonstrating a higher level of seric IgG to h-MM antigen in chronic compared to acute PCM patients.en
dc.description.affiliationUEL, UNOPAR, Biol & Hlth Ctr, Londrina, Brazil
dc.description.affiliationUEL, Microbiol Postgrad Program, Londrina, Brazil
dc.description.affiliationAdolfo Lutz Inst, Immunol Sect, São Paulo, Brazil
dc.description.affiliationState Univ Londrina, CCB, Dept Pathol Sci, Londrina, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent199-204
dc.identifierhttp://dx.doi.org/10.1002/jcla.20078
dc.identifier.citationJournal of Clinical Laboratory Analysis. Hoboken: Wiley-liss, v. 19, n. 5, p. 199-204, 2005.
dc.identifier.doi10.1002/jcla.20078
dc.identifier.issn0887-8013
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28070
dc.identifier.wosWOS:000232330900005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Clinical Laboratory Analysis
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectParacoccidioides brasiliensisen
dc.subjectparacoccidioidomycosisen
dc.subjecthigh molecular mass antigenen
dc.subjectIgGen
dc.subjectIgEen
dc.titleReactivity of antibodies from patients with acute and chronic paracoccidioidomycosis to a high molecular mass antigen from Paracoccidioides brasiliensisen
dc.typeinfo:eu-repo/semantics/article
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