CHARACTERIZATION OF A PHOSPHOLIPASE C-RESISTANT INOSITOL CONTAINING GLYCOLIPID FROM TRYPANOSOMA-CRUZI

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dc.contributor.authorHeise, Norton [UNIFESP]
dc.contributor.authorRaper, J.
dc.contributor.authorCardosodealmeida, M. L.
dc.contributor.institutionJOHNS HOPKINS UNIV
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T14:28:06Z
dc.date.available2018-06-15T14:28:06Z
dc.date.issued1994-02-01
dc.description.abstractSince glycosylphosphatidylinositol is the most common form of attachment of proteins to membranes in T. cruzi, and that this parasite depends on surface-mediated interactions for survival within the vector and mammalian host, it is probable that a drug which interfers with the metabolism of glycosylphosphatidylinositol (GPI) could be successfully employed in chemotherapy. Over the last few years several groups have been characterizing this mode of attachment in T. cruzi and more recently we have been concentrating our efforts on the identification of candidate precursors for protein anchors in metacyclic trypomastigotes. Previously detected GPI heterogeneity regarding solubilization of a major stage-specific antigen (1G7-Ag) by phospholipase C led us to investigate whether biosynthetic precursors with similar properties could also be identified. Two glycolipid species whose migration properties resemble glycolipids A and C of T. brucei were amenable to biosynthetic radiolabelling with palmitic acid, inositol, ethanolamine, glucosamine and mannose. Following purification,these species were submitted to classical GPI diagnostic treatments. In both cases digestion with GPI-specific phospholipase D (GPIPLD) produced phospatidic acid and treatment with either mild base or phospholipase A(2) (PLA(2)) produced free fatty acid, indicating an acylation at least at position 2 of the glycerol. The glycolipid A-like species proved to be susceptible to solubilization by PIPLC of B. thuringiensis and by GPIPLC of T. brucei and the glycolipid C-like material proved to be fully resistant to both lipases. Although the glycolipid A-like species indeed presents these and other properties compatible with a precursor for the chemically characterized 1 G7-Ag anchor, the PLC-resistant species which is completely insensitive to nitrous acid deamination might be an exception to the general finding of a non-acetylated glucosamine in the GPI moieties so far described.en
dc.description.affiliationJOHNS HOPKINS UNIV,DEPT BIOL CHEM,BALTIMORE,MD 21205
dc.description.sourceWeb of Science
dc.format.extent233-238
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 27, n. 2, p. 233-238, 1994.
dc.identifier.issn0100-879X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43051
dc.identifier.wosWOS:A1994MX60900018
dc.language.isoeng
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectGPIen
dc.subjectPHOSPHOLIPASE Cen
dc.subjectTRYPANOSOMA-CRUZIen
dc.subjectINOSITOL GLYCOLIPIDSen
dc.titleCHARACTERIZATION OF A PHOSPHOLIPASE C-RESISTANT INOSITOL CONTAINING GLYCOLIPID FROM TRYPANOSOMA-CRUZIen
dc.typeinfo:eu-repo/semantics/article
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