No mutations found in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide

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dc.contributor.authorMinicucci, Eliana Maria
dc.contributor.authorSilva, Glenda Nicioli da
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorFavero Salvadori, Daisy Maria
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T13:52:14Z
dc.date.available2016-01-24T13:52:14Z
dc.date.issued2009-02-01
dc.description.abstractThe medium-term tongue carcinogenesis assay is a useful model for studying oral squamous cell carcinomas phase by phase. the present study aimed to investigate mutations in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO) using direct DNA-sequencing method. A total of 30 male Wistar rats were treated with 4-nitroquinoline 1-oxide (4NQO) in drinking water for 4, 12, and 20 weeks at 50 ppm dose. Ten animals were used as negative control. No histopathological changes in tongue epithelia were observed among controls or in the group treated for 4 weeks with 4NQO. Following 12-week treatment, hyperplasia and epithelial dysplasia were found in mild and moderate forms. At 20 weeks, the tongue presented moderate and/or severe oral dysplasia and squamous cell carcinoma, with squamous cell carcinoma in the majority of animals. No mutations were found in any experimental period evaluated that corresponded to normal oral mucosa, hyperplasia, dysplasia and squamous cell carcinomas. Taken together, our results suggest that p16CDKN2A mutations in exon 2 are not involved in the multistep tongue carcinogenesis of Wistar rats induced by 4NQO.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biosci, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUNESP, Dept Dermatol & Radiotherapy, Botucatu, SP, Brazil
dc.description.affiliationUNESP, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biosci, BR-11060001 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 05/59156-3
dc.description.sponsorshipIDFAPESP: 07/01228-4
dc.format.extent71-76
dc.identifierhttp://dx.doi.org/10.1007/s10735-009-9215-4
dc.identifier.citationJournal of Molecular Histology. Dordrecht: Springer, v. 40, n. 1, p. 71-76, 2009.
dc.identifier.doi10.1007/s10735-009-9215-4
dc.identifier.issn1567-2379
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31282
dc.identifier.wosWOS:000264485300008
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Molecular Histology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectp16en
dc.subjectRat tongue mucosaen
dc.subjectOral squamous cell carcinomaen
dc.subject4-Nitroquinoline 1-oxideen
dc.subjectDirect DNA-sequencingen
dc.titleNo mutations found in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxideen
dc.typeinfo:eu-repo/semantics/article
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