Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes

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dc.citation.volumev. 17
dc.contributor.authorCruz, Maysa Mariana [UNIFESP]
dc.contributor.authorLopes, Andressa B.
dc.contributor.authorCrisma, Amanda R.
dc.contributor.authorSa, Roberta C. C. de [UNIFESP]
dc.contributor.authorKuwabara, Wilson M. T.
dc.contributor.authorCuri, Rui
dc.contributor.authorAndrade, Paula B. M. de
dc.contributor.authorVale, Maria Isabel Cardoso Alonso [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-07-20T16:31:14Z
dc.date.available2020-07-20T16:31:14Z
dc.date.issued2018
dc.description.abstractBackground: We have recently demonstrated that palmitoleic acid (16:1n7) increases lipolysis, glucose uptake and glucose utilization for energy production in white adipose cells. In the present study, we tested the hypothesis that palmitoleic acid modulates bioenergetic activity in white adipocytes. Methods: For this, 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes in the presence (or absence) of palmitic (16:0) or palmitoleic (16:1n7) acid at 100 or 200 mu M. The following parameters were evaluated:lipolysis, lipogenesis, fatty acid (FA) oxidation, ATP content, oxygen consumption, mitochondrial mass, citrate synthase activity and protein content of mitochondrial oxidative phosphorylation (OXPHOS) complexes. Results: Treatment with 16:1n7 during 9 days raised basal and isoproterenol-stimulated lipolysis, FA incorporation into triacylglycerol (TAG), FA oxidation, oxygen consumption, protein expression of subunits representing OXPHOS complex II, III, and V and intracellular ATP content. These effects were not observed in adipocytes treated with 16:0. Conclusions: Palmitoleic acid, by concerted action on lipolysis, Gamma A esterification, mitochondrial Gamma A oxidation, oxygen consumption and ATP content, does enhance white adipocyte energy expenditure and may act as local hormone.en
dc.description.affiliationUniv Fed Sao Paulo, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, 210 Sao Nicolau St, BR-09913030 Diadema, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, Brazil
dc.description.affiliationCruzeiro do Sul Univ, Inst Phys Act Sci & Sports, Interdisciplinary Postgrad Program Hlth Sci, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Espirito Santo, Hlth Sci Ctr, Dept Nursing, Vitoria, Spain
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, 210 Sao Nicolau St, BR-09913030 Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 2011/51627-8
dc.description.sponsorshipIDFAPESP: 2011/51701-3
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s12944-018-0710-z
dc.identifier.citationLipids In Health And Disease. London, v. 17, 2018.
dc.identifier.doi10.1186/s12944-018-0710-z
dc.identifier.fileWOS000427889100001.pdf
dc.identifier.issn1476-511X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55807
dc.identifier.wosWOS:000427889100001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofLipids In Health And Disease
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLipogenesisen
dc.subjectLipolysisen
dc.subjectTriglyceride/fatty acid cycleen
dc.subjectBioenergeticsen
dc.subjectMitochondriaen
dc.subjectBeta-oxidationen
dc.titlePalmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytesen
dc.typeinfo:eu-repo/semantics/article
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