Beneficial effect of low-level laser therapy in acute lung injury after i-I/R is dependent on the secretion of IL-10 and independent of the TLR/MyD88 signaling

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dc.citation.issue2
dc.citation.volume32
dc.contributor.authorCarvalho, Jorge Luis Costa [UNIFESP]
dc.contributor.authorBrito, Auriléia Aparecida de
dc.contributor.authorLigeiro de Oliveira, Ana Paula
dc.contributor.authorCastro-Faria-Neto, Hugo Caire
dc.contributor.authorAlbertini, Regiane [UNIFESP]
dc.contributor.authorAnatriello, Elen [UNIFESP]
dc.contributor.authorAimbire, Flavio [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-07-17T14:03:09Z
dc.date.available2020-07-17T14:03:09Z
dc.date.issued2017
dc.description.abstractThe use of low-level laser for lung inflammation treatment has been evidenced in animal studies as well as clinical trials. The laser action mechanism seems to involve downregulation of neutrophil chemoattractants and transcription factors. Innate immune responses against microorganisms may be mediated by toll-like receptors (TLR). Intestinal ischemia and reperfusion (i-I/R) lead to bacterial product translocation, such as endotoxin, which consequently activates TLRs leading to intestinal and lung inflammation after gut trauma. Thus, the target of this study was to investigate the role of TLR activation in the laser (660 nm, 30 mW, 67.5 J/cm(2), 0.375 mW/cm(2), 5.4 J, 180 s, and spot size with 0.08 cm(2)) effect applied in contact with the skin on axillary lymph node in lung inflammation induced by i-I/R through a signaling adaptor protein known as myeloid differentiation factor 88 (MyD88). It is a quantitative, experimental, and laboratory research using the C57Bl/6 and MyD88(-/-) mice (n = 6 mice for experimental group). Statistical differences were evaluated by ANOVA and the Tukey-Kramer multiple comparisons test to determine differences among groups. In order to understand how the absence of MyD88 can interfere in the laser effect on lung inflammation, MyD88(-/-) mice were treated or not with laser and subjected to occlusion of the superior mesenteric artery (45 min) followed by intestinal reperfusion (4 h). In summary, the laser decreased the MPO activity and the lung vascular permeability, thickened the alveolar septa, reduced both the edema and the alveolar hemorrhage, as well as significantly decreased neutrophils infiltration in MyD88-deficient mice as well in wild-type mice. It noted a downregulation in chemokine IL-8 production as well as a cytokine IL-10 upregulation in these animals. The results also evidenced that in absence of IL-10, the laser effect is reversed. Based on these results, we suggest that the beneficial effect of laser in acute lung injury after i-I/R is dependent on the secretion of IL-10 and independent of the TLR/MyD88 signaling.en
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Sci & Technol, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Nove de Julho UNINOVE, Lab Pulm Immunol & Exercise, Sao Jose Dos Campos, Brazil
dc.description.affiliationOswaldo Cruz Fdn FIOCRUZ, Lab Immunopharmacol, Rio De Janeiro, RJ, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Sci & Technol, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.format.extent305-315
dc.identifierhttps://dx.doi.org/10.1007/s10103-016-2115-4
dc.identifier.citationLasers In Medical Science. London, v. 32, n. 2, p. 305-315, 2017.
dc.identifier.doi10.1007/s10103-016-2115-4
dc.identifier.issn0268-8921
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55188
dc.identifier.wosWOS:000394154600007
dc.language.isoeng
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers In Medical Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectIntestinal ischemia-reperfusionen
dc.subjectLung inflammationen
dc.subjectMyeloid differentiation gene 88en
dc.subjectLaser therapyen
dc.titleBeneficial effect of low-level laser therapy in acute lung injury after i-I/R is dependent on the secretion of IL-10 and independent of the TLR/MyD88 signalingen
dc.typeinfo:eu-repo/semantics/article
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