Phylogenetic, morphological and behavioural analyses support host switching of Trypanosoma (Herpetosoma) lewisi from domestic rats to primates

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dc.contributor.authorSilva, F. Maia da
dc.contributor.authorMarcili, A.
dc.contributor.authorOrtiz, P. A.
dc.contributor.authorEpiphanio, S. [UNIFESP]
dc.contributor.authorCampaner, M.
dc.contributor.authorCatao-Dias, J. L.
dc.contributor.authorShaw, J. J.
dc.contributor.authorCamargo, E. P.
dc.contributor.authorTeixeira, M. M. G.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:59:39Z
dc.date.available2016-01-24T13:59:39Z
dc.date.issued2010-05-01
dc.description.abstractWe characterized four Brazilian trypanosomes isolated from domestic rats and three from captive nonhuman primates that were morphologically similar to T. lewisi, a considered non-pathogenic species restricted to rodents and transmitted by fleas, despite its potential pathogenicity for infants. These isolates were identified as T. lewisi by barcoding using V7V8 SSU rDNA sequences. in inferred phylogenetic trees, all isolates clustered tightly with reference T. lewisi and T. lewisi-like trypanosomes from Europe, Asia and Africa and despite their high sequence conservation formed a homogeneous clade separate from other species of the subgenus T. (Herpetosoma). With the aim of clearly resolving the relationships between the Brazilian isolates from domestic rats and primates, we compared sequences from more polymorphic ITS rDNA. Results corroborated that isolates from Brazilian rats and monkeys were indeed of the same species and quite close to T. lewisi isolates of humans and rats from different geographical regions. Morphology of the monkey isolates and their behaviour in culture and in experimentally infected rats were also compatible with T. lewisi. However, infection with T. lewisi is rare among monkeys. We have examined more than 200 free-ranging and 160 captive monkeys and found only three infected individuals among the monkeys held in captivity. the findings of this work suggest that proximity of monkeys and infected rats and their exposure to infected fleas may be responsible for the host switching of T. Iewisi from their natural rodent species to primates. This and previous studies reporting T. lewisi in humans suggest that this trypanosome can cause sporadic and opportunistic fleaborne infection in primates. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Dept Parasitol, Inst Biomed Sci, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Vet & Zootecnia, Dept Patol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent522-529
dc.identifierhttp://dx.doi.org/10.1016/j.meegid.2010.02.005
dc.identifier.citationInfection Genetics and Evolution. Amsterdam: Elsevier B.V., v. 10, n. 4, p. 522-529, 2010.
dc.identifier.doi10.1016/j.meegid.2010.02.005
dc.identifier.issn1567-1348
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32515
dc.identifier.wosWOS:000278629900014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInfection Genetics and Evolution
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosome lewisien
dc.subjectMonkeysen
dc.subjectPrimatesen
dc.subjectOpportunist infectionen
dc.subjectHost switchingen
dc.subjectMolecular diagnosisen
dc.subjectPhylogenetic relationshipsen
dc.titlePhylogenetic, morphological and behavioural analyses support host switching of Trypanosoma (Herpetosoma) lewisi from domestic rats to primatesen
dc.typeinfo:eu-repo/semantics/article
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