Canine paracoccidioidomycosis: a seroepidemiologic study

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dc.contributor.authorOno, Mario Augusto [UNIFESP]
dc.contributor.authorBracarense, Ana Paula Frederico Rodrigues Loureiro
dc.contributor.authorMorais, Helio Silva Autran de
dc.contributor.authorTrapp, Sílvia Manduca
dc.contributor.authorBelitardo, Donizeti Rodrigues
dc.contributor.authorCamargo, Zoilo Pires [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionDept Clin Vet
dc.date.accessioned2016-01-24T12:31:25Z
dc.date.available2016-01-24T12:31:25Z
dc.date.issued2001-06-01
dc.description.abstractSera from 305 dogs were analyzed by enzyme-linked immunosorbent assay (ELISA) to determine presence of the antibody anti-gp43, which reacts to a specific antigen of Paracoccidioides brasiliensis. the dogs were divided into three groups according to their origin: urban dogs (animals with little or no contact with rural areas); suburban dogs (from the urban outskirts); and rural dogs. There was a significant difference between groups (P <0.05). Rural dogs reacted positively in 89.5% of cases, followed by suburban (48.8%) and urban dogs (14.8%). There were no differences between male and female dogs. in an attempt to verify the feasibility of skin testing with gp43 to determine sensitization against P. brasiliensis in dogs, suburban (n = 61) and rural (n = 21) dogs were tested, showing positivity of 13.1 and 38.1%, respectively, Six dogs that had higher ELISA titers and also showed strong reactions in skin testing were killed in an attempt to isolate P, brasiliensis. the fungus was not detected by culture or histopathological analysis in these dogs, suggesting that dogs have a natural resistance or that they encounter an inoculum level that is insufficient to cause disease. These results indicate that ELISA and skin testing can be useful in the epidemiological study of paracoccidioidomycosis in dogs and that encounter with the fungus in nature is a frequent event.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Biol Celular, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Ciencias Patol, Londrina, Brazil
dc.description.affiliationDept Clin Vet, Londrina, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista de Medicina, Disciplina de Biologia Celular, Rua Botucatu, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent277-282
dc.identifierhttp://dx.doi.org/10.1080/mmy.39.3.277.282
dc.identifier.citationMedical Mycology. Oxford: B I O S Scientific Publishers Ltd, v. 39, n. 3, p. 277-282, 2001.
dc.identifier.doi10.1080/mmy.39.3.277.282
dc.identifier.issn1369-3786
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26580
dc.identifier.wosWOS:000169781300006
dc.language.isoeng
dc.publisherB I O S Scientific Publishers Ltd
dc.relation.ispartofMedical Mycology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectdogen
dc.subjectELISAen
dc.subjectParacoccidioides brasiliensisen
dc.subjectskin testen
dc.titleCanine paracoccidioidomycosis: a seroepidemiologic studyen
dc.typeinfo:eu-repo/semantics/article
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