rBmTI-6, a Kunitz-BPTI domain protease inhibitor from the tick Boophilus microplus, its cloning, expression and biochemical characterization

Página do item simplificado
dc.contributor.authorSasaki, Sergio D.
dc.contributor.authorTanaka, Aparecida S. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.date.accessioned2016-01-24T13:51:36Z
dc.date.available2016-01-24T13:51:36Z
dc.date.issued2008-08-01
dc.description.abstractBoophilus microplus is a rich source of trypsin inhibitors, numerous Kunitz-BPTI (bovine pancreatic trypsin inhibitor) inhibitors have been described from larvae and eggs, named BmTIs. Among them, were characterized inhibitors for trypsin, human neutrophil elastase, human plasma kallikrein and plasmin. BmTIs elicited a protective immunological response against B. microplus infestation in cattle. However, only a small amount of purified natural BmTIs can be obtained from larvae and eggs by chromatographic methods, thus if BmTIs are to be used as vaccine antigens (immunogens) the production of recombinant BmTIs (rBmTIs) is essential. in this work we describe the cloning, expression, purification and characterization of rBmTI-6. rBmTI-6 is a three-headed Kunitz-BPTI inhibitor, expressed in the Pichia pastoris system. Although rBmTI-6 was processed by proteases and glycosylated during the expression process, these post-translational modifications did not alter the ability of rBmTI-6 to inhibit protease activity. Purified rBmTI-6 inhibited trypsin and plasmin. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Fed Abc, Ctr Ciencias Nat & Humanas, BR-09090400 Santo Andre, SP, Brazil
dc.description.affiliationUnifespUNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent133-141
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2008.03.031
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 155, n. 1-2, p. 133-141, 2008.
dc.identifier.doi10.1016/j.vetpar.2008.03.031
dc.identifier.issn0304-4017
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30825
dc.identifier.wosWOS:000257980600018
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectticken
dc.subjectBoophilus microplusen
dc.subjectprotease inhibitoren
dc.subjecttrypsin inhibitoren
dc.subjectKunitzen
dc.titlerBmTI-6, a Kunitz-BPTI domain protease inhibitor from the tick Boophilus microplus, its cloning, expression and biochemical characterizationen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos