Protein tyrosine phosphatase alpha regulates cell detachment and cell death profiles induced by nitric oxide donors in the A(431) human carcinoma cell line

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dc.contributor.authorCosta, Paulo E. da [UNIFESP]
dc.contributor.authorBatista, Wagner L. [UNIFESP]
dc.contributor.authorCurcio, Marli F. [UNIFESP]
dc.contributor.authorMoraes, Miriam S. [UNIFESP]
dc.contributor.authorBorges, Roberta Eller [UNIFESP]
dc.contributor.authorNascimento, Patricia A. [UNIFESP]
dc.contributor.authorTravassos, Luiz R. [UNIFESP]
dc.contributor.authorMonteiro, Hugo P. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:06:02Z
dc.date.available2016-01-24T14:06:02Z
dc.date.issued2011-01-01
dc.description.abstractWe investigated the role of protein tyrosine phosphatase-alpha (PTP alpha) expression in the cell death profile of the A431 human carcinoma cell line that was induced by cytotoxic concentrations of the nitric oxide (NO) donors sodium nitroprusside (SNP) and 3,3-bis-(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18). Both NO donors promoted extensive cell detachment in A431 parental cells as compared to the detachment observed for A431 cells that ectopically expressed PTP alpha (A431 (A27B(PTP alpha)) cells). the NO-induced cell death characteristics for both cell lines were examined. After incubation for 10 hours with 2.0 mM SNP, attached or detached A431 cells underwent apoptosis. Cells were highly positive for Annexin-V, featured increased cleavage of procaspase-8, activation of downstream caspase-3, and activation of poly-ADP-ribose polymerase 1 (PARP-1). in contrast, exposure of A431 (A27B(PTP alpha)) cells to 2.0 mM SNP produced an increase in the release of lactate dehydrogenase and enhanced incorporation of propidium iodide. in addition, A431 (A27B(PTP alpha)) cells showed partial inhibition of the activities of caspase-8, caspase-3, and PARP-1 upon detachment and cell death induced by SNP treatment. Results indicate that necrotic cell damage was induced, characterized by cellular swelling and lysis. We conclude from these results that PTP alpha regulates the A431 tumor cell death profile mediated by NO donors. Expression of PTP alpha or its absence may determine the occurrence of NO-induced cell death with necrotic or apoptotic features, respectively.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem Mol Biol, Ctr Cellular & Mol Therapy CTCMol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Clin Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem Mol Biol, Ctr Cellular & Mol Therapy CTCMol, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Clin Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 06/56311-0
dc.description.sponsorshipIDFAPESP: 07/59617-6
dc.description.sponsorshipIDCNPq: 42011/2005
dc.format.extent27-37
dc.identifierhttp://dx.doi.org/10.1179/174329211X12968219310792
dc.identifier.citationRedox Report. Leeds: Maney Publishing, v. 16, n. 1, p. 27-37, 2011.
dc.identifier.doi10.1179/174329211X12968219310792
dc.identifier.issn1351-0002
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33341
dc.identifier.wosWOS:000290614000005
dc.language.isoeng
dc.publisherManey Publishing
dc.relation.ispartofRedox Report
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectA431 tumor cellsen
dc.subjectCell deathen
dc.subjectEpidermal growth factoren
dc.subjectNitric oxide donorsen
dc.subjectProtein tyrosine phosphatase-alphaen
dc.subjectSodium nitroprussideen
dc.titleProtein tyrosine phosphatase alpha regulates cell detachment and cell death profiles induced by nitric oxide donors in the A(431) human carcinoma cell lineen
dc.typeinfo:eu-repo/semantics/article
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