Histopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbits

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dc.contributor.authorSouza, Geovanne F. [UNIFESP]
dc.contributor.authorCalado, Adriano A. [UNIFESP]
dc.contributor.authorDelcelo, Rosana [UNIFESP]
dc.contributor.authorOrtiz, Valdemar [UNIFESP]
dc.contributor.authorMacedo Jr., Antonio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:38:37Z
dc.date.available2015-06-14T13:38:37Z
dc.date.issued2008-06-01
dc.description.abstractPURPOSE: Buccal mucosa is a widely accepted tissue for urethroplasty. The exact healing and tissue integration process, mainly the histological characteristics of dorsal buccal mucosa graft urethroplasty when used dorsally to reconstruct the urethral plate has not previously been assessed, and thus we developed an experimental model to address this question. MATERIALS AND METHODS: In 12 New Zealand rabbits (weight 2.5 kg) we surgically created a dorsal penile urethral defect. A buccal mucosa graft was sutured to the corpora and tunica albuginea, and the ventral urethra anastomosed to this new urethral plate. The animals were divided in three groups and sacrificed 1, 3 and 6 weeks after surgery (groups 1, 2 and 3). A retrograde urethrogram was obtained at autopsy in the last group and the penis analyzed histologically with hematoxylin-eosin and Masson's staining. RESULTS: The urethrograms showed no evidence of fistula or stricture. In group 1 the histopathological analysis showed submucosal lymph-mononuclear inflammatory edema, numerous eosinophils and squamous epithelium integrated into the adjacent urothelium. In group 2 there was no evidence of an inflammatory response but rather complete subepithelial hyaline healing, which was more marked in group 3. CONCLUSION: Healing of buccal mucosa grafts to reconstruct the urethral plate can be achieved by total integration of the squamous epithelium with the urothelium, maintaining the original histological properties of the graft with no fibrosis or retraction.en
dc.description.affiliationFederal University of São Paulo Divisions of Urology
dc.description.affiliationFederal University of São Paulo
dc.description.affiliationUnifespUNIFESP, Divisions of Urology
dc.description.affiliationUnifespUNIFESP
dc.description.sourceSciELO
dc.format.extent345-354
dc.identifierhttp://dx.doi.org/10.1590/S1677-55382008000300012
dc.identifier.citationInternational braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 3, p. 345-354, 2008.
dc.identifier.doi10.1590/S1677-55382008000300012
dc.identifier.fileS1677-55382008000300012.pdf
dc.identifier.issn1677-5538
dc.identifier.scieloS1677-55382008000300012
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/4451
dc.identifier.wosWOS:000207704700027
dc.language.isoeng
dc.publisherSociedade Brasileira de Urologia
dc.relation.ispartofInternational braz j urol
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecturethraen
dc.subjectbuccal mucosaen
dc.subjectrabbitsen
dc.subjectexperimentalen
dc.subjectsurgeryen
dc.titleHistopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbitsen
dc.typeinfo:eu-repo/semantics/article
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