Genotoxic assessment of Rubus imperialis (Rosaceae) extract in vivo and its potential chemoprevention against cyclophosphamide-induced DNA damage

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dc.contributor.authorCosta Rodrigues Alves, Ana Beatriz
dc.contributor.authorSantos, Rafaella Souza dos
dc.contributor.authorCalil, Susana de Santana
dc.contributor.authorNiero, Rivaldo
dc.contributor.authorLopes, Jhonny da Silva
dc.contributor.authorPerazzo, Fabio E. [UNIFESP]
dc.contributor.authorPires Rosa, Paulo Cesar [UNIFESP]
dc.contributor.authorAndrade, Sergio Faloni
dc.contributor.authorCechinel-Filho, Valdir
dc.contributor.authorMaistro, Edson Luis
dc.contributor.institutionUniv Estadual Paulista
dc.contributor.institutionUniv Vale Itajai
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:37:17Z
dc.date.available2016-01-24T14:37:17Z
dc.date.issued2014-05-14
dc.description.abstractEthnopharmacological relevance: Rubus imperialis Cham. Schl. (Rosaceae) is frequently used in traditional medicine as hypoglycemic, antinociceptive and antiviral remedy.Materials and methods: Swiss albino mice were distributed in eight groups for acute treatment with Rubus imperialis extract (24 h). the extract doses selected were 50, 250 and 500 mg/kg b.w. administered by gavage alone or plus to CPA (50 mg/kg b.w.) administered by intraperitoneal injection. Control groups were treated in a similar way. Analyses were performed using the comet assay, on leukocytes (collected 4 and 24 h after treatment) and liver (collected 24 h after treatment), and using the micronucleus test (MN) in bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio).Results and conclusion: the main compounds identified in the Rubus imperialis extract were saponins and steroidal compounds, with niga-ichigoside and tormentic acid being the major compounds. Tested doses of Rubus imperialis extract showed no genotoxic effects on leukocytes from peripheral blood or liver cells by the comet assay. However, the MN test showed an increase in the frequency of micronucleated cells at the two higher doses tested, indicating that this extract has clastogenic/aneugenic effects on bone marrow cells at higher doses. On the other hand, for all cells evaluated, the three tested doses of the Rubus imperialis extract promoted inhibition of DNA damage induced by CPA. Despite the chemoprevention observed, the clastogenicity/aneugenicity observed suggested caution about either continuous or high-dose usage of Rubus imperiale aerial parts extract by humans. (C) 2014 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Fac Filosofia & Ciencias, Dept Fonoaudiol, UNESP, BR-17525900 São Paulo, Brazil
dc.description.affiliationUniv Vale Itajai, Nucleo Invest Quim Farmaceut NIQFAR, Programa Posgrad Ciencias Farmaceut, UNIVALI, Itajai, SC, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, UNIFESP, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Exatas & Terra, UNIFESP, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipUNESP, Marilia, Brazil
dc.description.sponsorshipIDFAPESP: 2010/07577-3
dc.format.extent694-700
dc.identifierhttp://dx.doi.org/10.1016/j.jep.2014.03.033
dc.identifier.citationJournal of Ethnopharmacology. Clare: Elsevier B.V., v. 153, n. 3, p. 694-700, 2014.
dc.identifier.doi10.1016/j.jep.2014.03.033
dc.identifier.issn0378-8741
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37768
dc.identifier.wosWOS:000337648800018
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Ethnopharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectRubus imperialisen
dc.subjectRosaceaeen
dc.subjectComet assayen
dc.subjectMicronucleus testen
dc.subjectAntigenotoxic effectsen
dc.titleGenotoxic assessment of Rubus imperialis (Rosaceae) extract in vivo and its potential chemoprevention against cyclophosphamide-induced DNA damageen
dc.typeinfo:eu-repo/semantics/article
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