MMP-9 Expression Increases According to the Grade of Squamous Intraepithelial Lesion in Cervical Smears

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dc.contributor.authorMatheus, Erika R.
dc.contributor.authorZonta, Marco A.
dc.contributor.authorDiscacciati, Michelle G.
dc.contributor.authorParuci, Priscila
dc.contributor.authorVelame, Fernanda
dc.contributor.authorCardeal, Laura B. S.
dc.contributor.authorBarros, Silvia B. M.
dc.contributor.authorPignatari, Antonio C. [UNIFESP]
dc.contributor.authorMaria-Engler, Silvya S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionIn Cito Lab Diagnost Cytol
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:37:54Z
dc.date.available2016-01-24T14:37:54Z
dc.date.issued2014-10-01
dc.description.abstractStudies about cervical carcinogenesis have demonstrated the increased expression of matrix-metalloproteinase (MMP) according to the grade of cervical intraepithelial lesions. Considering the importance of innovative techniques to introduce noninvasive and rapid diagnoses for patients, this study aimed to perform MMP-9 immunocytochemistry in cervical smears according to the cytopathological diagnoses, in order to monitor MMP activity in cervical smears. This cross-sectional study investigated the expression of MMP-9 in normal cervical smears, inflammatory cervical smears, squamous intraepithelial lesions, and cervical carcinoma. Cervical smears from 630 women were collected for cytopathological diagnoses and immunocytochemistry. Women with squamous intraepithelial lesions showed an increase in MMP-9 expression, with moderate to intense staining occurring with increasing cervical lesion grade. the prevalence of moderate to intense MMP-9 staining was 9% in normal cervical smears, 12% in cervical inflammation, 24% in low-grade squamous intraepithelial lesion (LSIL), 92% in high-grade squamous intraepithelial lesions (HSIL) and 100% in cervical carcinoma cases. in the specific case of LSIL, we found that association with MMP-9 is more evident when there is the simultaneous presence of an infectious agent. Thus, the expression of MMP-9 in cervical smears increases according to the grade of cervical lesion and LSIL in the presence of infectious agents showed higher MMP-9 expression than women with LSIL without infectious agents. Diagn. Cytopathol. 2014;42:827-833. (c) 2014 Wiley Periodicals, Inc.en
dc.description.affiliationUniv São Paulo, Sch Pharmaceut Sci, Dept Clin Chem & Toxicol, Lab Clin Pathol & Cytol, BR-05508000 São Paulo, Brazil
dc.description.affiliationIn Cito Lab Diagnost Cytol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, Special Lab Clin Microbiol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, Special Lab Clin Microbiol, BR-05508000 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPro-Reitoria de Pos-Graduacao da Universidade de São Paulo (PRPG-USP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipINCT_if
dc.description.sponsorshipIDFAPESP: 2008/58817-4
dc.description.sponsorshipIDFAPESP: 2012/09746-2
dc.description.sponsorshipIDINCT_if: 573663/2008-4
dc.format.extent827-833
dc.identifierhttp://dx.doi.org/10.1002/dc.23124
dc.identifier.citationDiagnostic Cytopathology. Hoboken: Wiley-Blackwell, v. 42, n. 10, p. 827-833, 2014.
dc.identifier.doi10.1002/dc.23124
dc.identifier.issn8755-1039
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38260
dc.identifier.wosWOS:000342690200001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofDiagnostic Cytopathology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectmatrix metalloproteinase 9en
dc.subjectcervical smearen
dc.subjectcervical canceren
dc.subjecthuman papillomavirusen
dc.subjectcervical intraepithelial neoplasiaen
dc.subjectgenital tract infectionsen
dc.subjectinflammationen
dc.subjectuterine cervical canceren
dc.subjectMMP-9en
dc.subjectpap smearen
dc.titleMMP-9 Expression Increases According to the Grade of Squamous Intraepithelial Lesion in Cervical Smearsen
dc.typeinfo:eu-repo/semantics/article
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