Neuromodulatory property of standardized extract Ginkgo biloba L. (EGb 761) on memory: Behavioral and molecular evidence

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dc.contributor.authorOliveira, Daniela R. [UNIFESP]
dc.contributor.authorSanada, Priscila F. [UNIFESP]
dc.contributor.authorSaragossa Filho, A. C. [UNIFESP]
dc.contributor.authorInnocenti, L. R.
dc.contributor.authorOler, Gisele [UNIFESP]
dc.contributor.authorCerutti, Janete M. [UNIFESP]
dc.contributor.authorCerutti, Suzete M. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Sao Francisco
dc.date.accessioned2016-01-24T13:52:32Z
dc.date.available2016-01-24T13:52:32Z
dc.date.issued2009-05-07
dc.description.abstractAlthough it has been suggested that the standardized Ginkgo biloba leaf extract (Egb 761) may have a beneficial effect on memory, the cellular and molecular changes that underlie this process are not yet well defined. the present study evaluated the effects of acute (one dose) or subacute treatments (one daily dose/seven days) with EGb 761 (0.5 g kg(-1) and 1.0 g kg(-1)) on rats submitted to a conditioned emotional response (CER) in comparison with positive (4 mg kg(-1) Diazepam) and negative (12% Tween 80) control groups. To this end, eighty (n = 10/group) adult, male, Wistar rats (+/- 250-300 g) were used in an off-baseline CER procedure. We here observed that the rats submitted to an acute and subacute EGb 761 treatments had acquisition of fear conditioning. Additionally, we investigate if the expression of genes previously associated with classical conditioning (CREB-1 and GAP-43) and new candidate genes (GFAP) are modulated following EGb 761 acute treatment. CREB-1, GAP-43 and GFAP mRNA and protein expressions were evaluated using both quantitative PCR (qPCR) and immunohistochemical analysis, respectively. We here show, for the first time, that EGb 761 modulated GAP-43, CREB-1 and GFAP expression in the prefrontal cortex, amygdala and hippocampus. We observed an underexpression of GAP-43 in all structures evaluated and over-expression of GFAP in the amygdala and hippocampus following acute G. biloba treatment when compared to control group (Tween; p<0.01). GAP-43 expression was decreased in prefrontal cortex and hippocampus in the subacute treatment with EGb 761. Subacute treatment with EGb 761 lead to a decreased CREB-1 in mPFC (p<0.001) and increased in the hippocampus to 1.0 g kg(-1) G. biloba group (p<0.001). the results obtained from immunohistochemical analysis support our aforementioned findings and revealed that the changes in expression occurred within specific regions in the areas evaluated. All together, our findings not only provide new evidence for a role of EGb 761 on memory but also identify molecular changes that underlie the fear memory consolidation. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, BR-09972270 Eldorado Diadema, SP, Brazil
dc.description.affiliationUniv Sao Francisco, Lab Neural Plast & Phytotherapy, BR-12900000 Braganca Paulista, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol, Genet Bases Thyroid Tumor Lab, Div Genet, BR-09972270 Eldorado Diadema, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, BR-09972270 Eldorado Diadema, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Genetic Bases of Thyroid Tumor Laboratory, Division of Genetics, Department of Morphology, Federal University of Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 02-09457-9
dc.description.sponsorshipIDFAPESP: 05-60330-8
dc.format.extent68-89
dc.identifierhttp://dx.doi.org/10.1016/j.brainres.2008.11.105
dc.identifier.citationBrain Research. Amsterdam: Elsevier B.V., v. 1269, p. 68-89, 2009.
dc.identifier.doi10.1016/j.brainres.2008.11.105
dc.identifier.issn0006-8993
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31516
dc.identifier.wosWOS:000266294900009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBrain Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectFear conditioningen
dc.subjectCREB-1en
dc.subjectGAP-43en
dc.subjectGFAPen
dc.subjectGinkgo bilobaen
dc.subjectLearning and memoryen
dc.titleNeuromodulatory property of standardized extract Ginkgo biloba L. (EGb 761) on memory: Behavioral and molecular evidenceen
dc.typeinfo:eu-repo/semantics/article
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