Frequency of Toxoplasma gondii in the retina in eye banks in Brazil

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dc.citation.issue7]
dc.citation.volume116]
dc.contributor.authorCosta, Deise F. [UNIFESP]
dc.contributor.authorNascimento, Heloisa [UNIFESP]
dc.contributor.authorSutili, Aline [UNIFESP]
dc.contributor.authorNobrega, Fernando A. J.
dc.contributor.authorFowler, Flavio [UNIFESP]
dc.contributor.authorNobrega, Mario Junqueira
dc.contributor.authorGarrido, Cristina
dc.contributor.authorDias, Janaina de Oliveira
dc.contributor.authorAdan, Consuelo B. D.
dc.contributor.authorRizzo, Luiz Vicente
dc.contributor.authorSilveira, Claudio
dc.contributor.authorBelfort, Rubens, Jr. [UNIFESP]
dc.contributor.authorCommodaro, Alessandra G. [UNIFESP]
dc.coverageNew York
dc.date.accessioned2020-06-26T16:30:32Z
dc.date.available2020-06-26T16:30:32Z
dc.date.issued2017
dc.description.abstractOcular toxoplasmosis is the main cause of posterior uveitis worldwide frequently leading to vision loss. In Brazil, the seroprevalence of Toxoplasma gondii infection ranges from 50 to 80% depending of the region studied. The frequency of toxoplasmic retinal scar may reach 18% of the adults in the South of Brazil. Our goal was to determine the frequency of T. gondii DNA in retinas from eye banks from different regions in Brazil. A total of 162 eyes were obtained from eye banks in Manaus (n = 60), Sao Paulo (n = 60), Chapeco (n = 26), and Joinville (n = 16). The retinas were macroscopically analyzed and collected for DNA extraction. Real-time PCR (qPCR) was performed using the T. gondii B1 marker. By qPCR, a higher frequency of T. gondii DNA in the retinas from the eye bank of Joinville (25%) was found when compared to Manaus (5%). The retinas from Sao Paulo and Chapeco were qPCR negative. Clinical examination determined the retina lesions to be compatible with toxoplasmosis in the following frequencies: Joinville (62.5%), Manaus (10%), Sao Paulo (6.7%), and Chapeco (15.4%).en
dc.description.affiliationUniv Fed Sao Paulo, Dept Ophthalmol, Rua Botucatu 821, BR-04023062 Sao Paulo, SP, Brazil
dc.description.affiliationVis Inst, Rua Botucatu 821, BR-04023062 Sao Paulo, SP, Brazil
dc.description.affiliationEye Bank Joinville, Joinville, SC, Brazil
dc.description.affiliationEye Bank Manaus, Manaus, Amazonas, Brazil
dc.description.affiliationEye Bank Chapeco, Chapeco, SC, Brazil
dc.description.affiliationEye Bank Sao Paulo, Sao Paulo, SP, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, Sao Paulo, Brazil
dc.description.affiliationClin Silveira, Erechim, RS, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ophthalmol, Rua Botucatu 821, BR-04023062 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCAPES
dc.description.sponsorshipIDCNPq: 400040/2014-0
dc.description.sponsorshipIDFAPESP
dc.description.sponsorshipIDCAPES
dc.format.extent2031-2033
dc.identifierhttp://dx.doi.org/10.1007/s00436-017-5474-2]
dc.identifier.citationParasitology Research. New York, v. 116, n. 7, p. 2031-2033, 2017.
dc.identifier.doi10.1007/s00436-017-5474-2
dc.identifier.issn0932-0113
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53609
dc.identifier.wosWOS:000403940600031
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofParasitology Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectEye banken
dc.subjectBrazilen
dc.subjectToxoplasma gondiien
dc.subjectReal-time PCRen
dc.titleFrequency of Toxoplasma gondii in the retina in eye banks in Brazilen
dc.typeinfo:eu-repo/semantics/article
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