Stimulation of renal afferent fibers leads to activation of catecholaminergic and non-catecholaminergic neurons in the medulla oblongata

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dc.citation.volume204
dc.contributor.authorNishi, Erika E.[UNIFESP]
dc.contributor.authorMartins, Beatriz S.[UNIFESP]
dc.contributor.authorMilanez, Maycon I. O.[UNIFESP]
dc.contributor.authorLopes, Nathalia R.[UNIFESP]
dc.contributor.authorde Melo, Jose F., Jr.[UNIFESP]
dc.contributor.authorPontes, Roberto B.
dc.contributor.authorGirardi, Adriana C.
dc.contributor.authorCampos, Ruy R.[UNIFESP]
dc.contributor.authorBergamaschi, Cassia T.[UNIFESP]
dc.coverageAmsterdam
dc.date.accessioned2020-07-13T11:53:25Z
dc.date.available2020-07-13T11:53:25Z
dc.date.issued2017
dc.description.abstractPresympathetic neurons in the rostral ventrolateral medulla (RVLM) including the adrenergic cell groups play a major role in the modulation of several reflexes required for the control of sympathetic vasomotor tone and blood pressure (BP). Moreover, sympathetic vasomotor drive to the kidneys influence natriuresis and diuresis by inhibiting the cAMP/PKA pathway and redistributing the Na+/H+ exchanger isoform 3 (NHE3) to the body of the microvilli in the proximal tubules. In this study we aimed to evaluate the effects of renal afferents stimulation on (1) the neurochemical phenotype of Fos expressing neurons in the medulla oblongata and (2) the level of abundance and phosphorylation of NHE3 in the renal cortex. We found that electrical stimulation of renal afferents increased heart rate and BP transiently and caused activation of tyrosine hydroxylase (TH)-containing neurons in the RVLM and non-TH neurons in the NTS. Additionally, activation of the inhibitory renorenal reflex over a 30-min period resulted in increased natriuresis and diuresis associated with increased phosphorylation of NHE3 at serine 552, a surrogate for reduced activity of this exchanger, in the contralateral kidney. This effect was not dependent of BP changes considering that no effects on natriuresis or diuresis were found in the ipsilateral-stimulated kidney. Therefore, our data show that renal afferents leads to activation of catecholaminergic and non-catecholaminergic neurons in the medulla oblongata. When renorenal reflex is induced, NHE3 exchanger activity appears to be decreased, resulting in decreased sodium and water reabsorption in the contralateral kidney. (C) 2017 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Physiol, Escola Paulista Med, Rua Botucatu 862, BR-04023060 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Med, Heart Inst InCor, Lab Genet & Mol Cardiol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Physiol, Escola Paulista Med, Rua Botucatu 862, BR-04023060 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCAPES-PNPD
dc.description.sponsorshipFAPESP undergraduate scholarship
dc.description.sponsorshipCNPq productive research fellowships
dc.description.sponsorshipIDFAPESP: 2013/22522-9
dc.description.sponsorshipIDFAPESP: 2013/23741-6
dc.description.sponsorshipIDFAPESP: 2015/23809-5
dc.description.sponsorshipIDFAPESP: 2015/23858-6
dc.format.extent48-56
dc.identifierhttp://dx.doi.org/10.1016/j.autneu.2017.01.003
dc.identifier.citationAutonomic Neuroscience-Basic & Clinical. Amsterdam, v. 204, p. 48-56, 2017.
dc.identifier.doi10.1016/j.autneu.2017.01.003
dc.identifier.issn1566-0702
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54615
dc.identifier.wosWOS:000400219400006
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofAutonomic Neuroscience-Basic & Clinical
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectRenal afferentsen
dc.subjectRVLMen
dc.subjectNTSen
dc.subjectTyrosine hydroxylaseen
dc.subjectNHE3en
dc.subjectRenorenal reflexen
dc.titleStimulation of renal afferent fibers leads to activation of catecholaminergic and non-catecholaminergic neurons in the medulla oblongataen
dc.typeinfo:eu-repo/semantics/article
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