Interactions between TLR2, TLR4, and mannose receptors with gp43 from Paracoccidioides brasiliensis induce cytokine production by human monocytes

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dc.contributor.authorNakaira-Takahagi, Erika
dc.contributor.authorGolim, Marjorie A.
dc.contributor.authorBannwart, Camila F.
dc.contributor.authorPuccia, Rosana [UNIFESP]
dc.contributor.authorPeraçoli, Maria Terezinha Serrão [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:15Z
dc.date.available2016-01-24T14:17:15Z
dc.date.issued2011-10-01
dc.description.abstractThe glycoprotein gp43 is an immunodominant antigen secreted by Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis. the present study evaluated whether gp43 can interact with toll-like (TLR2, TLR4) and mannose (MR) receptors on the surface of human monocytes, and how that affects their expression and cytokine production. Monocytes were incubated with or without monoclonal antibodies anti-TLR2, anti-TLR4, or anti-MR, individually or in combination, prior to the addition of gp43. the gp43 binding to monocyte surface, as well as expression of TLR2, TLR4, and MRs were analyzed by flow cytometry, while production of TNF-alpha and IL-10 was monitored by ELISA. the results suggested that gp43 binds to TLR2, TLR4, and MR receptors, with TLR2 and MR having the strongest effect. All three receptors influenced the production of IL-10, while TNF-alpha production was associated with expression of TLR4 and MR. the modulatory effect of gp43 was demonstrated by high levels of TLR4 expression associated with increased production of TNF-alpha after 4 h of culture. Alternatively, high levels of TLR2 expression, and elevated production of IL-10, were detected after 18 h. We showed that interaction between gp43 and monocytes may affect the innate immune response by modulating the expression of the pattern recognition receptors TLR2, TLR4 and MR, as well as production of pro- and anti-inflammatory cytokines.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Microbiol & Imunol, Inst Biociencias, BR-18618970 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Hemoctr, Fac Med, BR-18618970 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2006/53366-9
dc.format.extent694-703
dc.identifierhttp://dx.doi.org/10.3109/13693786.2011.565485
dc.identifier.citationMedical Mycology. London: Informa Healthcare, v. 49, n. 7, p. 694-703, 2011.
dc.identifier.doi10.3109/13693786.2011.565485
dc.identifier.issn1369-3786
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34082
dc.identifier.wosWOS:000294866400004
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofMedical Mycology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.subjectParacoccidioides brasiliensisen
dc.subjectgp43en
dc.subjectmonocytesen
dc.subjectToll-like receptorsen
dc.subjectmannose receptoren
dc.subjectTNF-alphaen
dc.subjectIL-10en
dc.titleInteractions between TLR2, TLR4, and mannose receptors with gp43 from Paracoccidioides brasiliensis induce cytokine production by human monocytesen
dc.typeinfo:eu-repo/semantics/article
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