Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis

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dc.contributor.authorMoreira Filho, Renato Evando
dc.contributor.authorBandeira, Silviane Praciano
dc.contributor.authorNogueira Brillhante, Raimunda Samia
dc.contributor.authorGadelha Rocha, Marcos Fabio
dc.contributor.authorVasconcelos, Ilka Maria
dc.contributor.authorPereira, Mirella Leite
dc.contributor.authorCollares Maia Castelo-Branco, Debora de Souza
dc.contributor.authorCastro Rocha, Francisco Airton
dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.authorRamos, Marcio Viana
dc.contributor.authorCordeiro, Rossana de Aguiar
dc.contributor.authorCosta Sidrim, Jose Julio
dc.contributor.institutionUniv Fed Ceara
dc.contributor.institutionUniv Estadual Ceara
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:24Z
dc.date.available2016-01-24T14:27:24Z
dc.date.issued2012-07-01
dc.description.abstractThe objective of this study was to evaluate the reactivity of an in-house antigen, extracted from a strain of C. posadasii isolated in northeastern Brazil, by radial immunodiffusion and Western blotting, as well as to establish its biochemical characterization. the protein antigen was initially extracted with the use of solid ammonium sulfate and characterized by 1-D electrophoresis. Subsequently, it was tested by means of double radial immunodiffusion and Western blotting. A positive reaction was observed against the antigen by both immunodiagnostic techniques tested on sera from patients suffering from coccidioidomycosis. Besides this, two immunoreactive protein bands were observed and were revealed to be a beta-glucosidase and a glutamine synthetase after sequencing of the respective N-terminal regions. Our in-house Coccidioides antigen can be promising as a quick and low-cost diagnostic tool without the risk of direct manipulation of the microorganism.en
dc.description.affiliationUniv Fed Ceara, Specialized Med Mycol Ctr, Sch Med, BR-60430270 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Estadual Ceara, Postgrad Program Vet Sci, Sch Vet Med, BR-60740000 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Biochem & Mol Biol, BR-60455760 Fortaleza, Ceara, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04021001 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04021001 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel
dc.description.sponsorshipIDCNPq: 302574/2009-3
dc.description.sponsorshipIDCNPq: 306637/2010-3
dc.description.sponsorshipIDCoordination for the Improvement of Higher Education Personnel: CAPES/PNPD2103/2009
dc.format.extent7854-7863
dc.identifierhttp://dx.doi.org/10.3390/molecules17077854
dc.identifier.citationMolecules. Basel: Mdpi Ag, v. 17, n. 7, p. 7854-7863, 2012.
dc.identifier.doi10.3390/molecules17077854
dc.identifier.fileWOS000306752700018.pdf
dc.identifier.issn1420-3049
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35024
dc.identifier.wosWOS:000306752700018
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofMolecules
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcoccidioidomycosisen
dc.subjectimmunodiagnosisen
dc.subjectantigensen
dc.subjectbeta-glucosidaseen
dc.subjectglutamineen
dc.titleBiochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosisen
dc.typeinfo:eu-repo/semantics/article
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