Cafestol, a diterpene molecule found in coffee, induces leukemia cell death

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dc.contributor.authorLimaa, Caue S. [UNIFESP]
dc.contributor.authorSpindola, Daniel G. [UNIFESP]
dc.contributor.authorBechara, Alexandre [UNIFESP]
dc.contributor.authorGarcia, Daniel M. [UNIFESP]
dc.contributor.authorPalmeira-dos-Santos, Caroline [UNIFESP]
dc.contributor.authorPeixoto-da-Silva, Janaina [UNIFESP]
dc.contributor.authorErustes, Adolfo G. [UNIFESP]
dc.contributor.authorMichelin, Luis F. G. [UNIFESP]
dc.contributor.authorPereira, Gustavo J. S. [UNIFESP]
dc.contributor.authorSmaili, Soraya S. [UNIFESP]
dc.contributor.authorParedes-Gamero, Edgar [UNIFESP]
dc.contributor.authorCalgarotto, Andrana K. [UNIFESP]
dc.contributor.authorOliveira, Carlos R. [UNIFESP]
dc.contributor.authorBincoletto, Claudia [UNIFESP]
dc.date.accessioned2019-08-19T11:49:58Z
dc.date.available2019-08-19T11:49:58Z
dc.date.issued2017
dc.description.abstractTo evaluate the antitumor properties of Cafestol four leukemia cell lines were used (NB4, K562, HL60 and KG1). Cafestol exhibited the highest cytotoxicity against HL60 and KG1 cells, as evidenced by the accumulation of cells in the sub-G1 fraction, mitochondrial membrane potential reduction, accumulation of cleaved caspase-3 and phosphatidylserine externalization. An increase in CD11b and CD15 differentiation markers with attenuated ROS generation was also observed in Cafestol-treated HL60 cells. These results were similar to those obtained following exposure of the same cell line to cytarabine (Ara-C), an antileukemic drug. Cafestol and Ara-C reduced the clonogenic potential of HL60 cells by 100%, but Cafestol spared murine colony forming unit-granulocyte/macrophage (CFU-GM), which retained their clonogenicity. The co-treatment of Cafestol and Ara-C reduced HL60 cell viability compared with both drugs administered alone. In conclusion, despite the distinct molecular mechanisms involved in the activity of Cafestol and Ara-C, a similar cytotoxicity towards leukemia cells was observed, which suggests a need for prophylactic-therapeutic pre-clinical studies regarding the anticancer properties of Cafestol. (C) 2017 Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniv Fed São Paulo, EPM, Dept Farmacol, São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo UNIFESP, EPM, Dept Bioquim, São Paulo, SP, Brazil
dc.description.affiliationUniv Anhembi Morumbi, Escola Ciencias Saude, Grp Fitocomplexos & Sinalizacao Celular, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo, EPM, Dept Farmacol, São Paulo, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo UNIFESP, EPM, Dept Bioquim, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) fellowship
dc.format.extent1045-1054
dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2017.05.109
dc.identifier.citationBiomedicine & Pharmacotherapy. Paris, v. 92, p. 1045-1054, 2017.
dc.identifier.doi10.1016/j.biopha.2017.05.109
dc.identifier.issn0753-3322
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51447
dc.identifier.wosWOS:000407915600117
dc.language.isoeng
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCafestolen
dc.subjectLeukemiaen
dc.subjectApoptosisen
dc.subjectCytotoxicityen
dc.subjectClonogenicityen
dc.titleCafestol, a diterpene molecule found in coffee, induces leukemia cell deathen
dc.typeinfo:eu-repo/semantics/article
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