A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential

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dc.contributor.authorBrito, Adriana S.
dc.contributor.authorCavalcante, Romulo S.
dc.contributor.authorPalhares, Lais C. G. F.
dc.contributor.authorHughes, Ashley J.
dc.contributor.authorAndrade, Giulianna P. V.
dc.contributor.authorYates, Edwin Alexander [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorLima, Marcelo Andrade de [UNIFESP]
dc.contributor.authorChavante, Suely F.
dc.contributor.institutionUniv Fed Rio Grande do Norte
dc.contributor.institutionUniv Liverpool
dc.contributor.institutionDiamond Light Source Ltd
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:08Z
dc.date.available2016-01-24T14:35:08Z
dc.date.issued2014-01-02
dc.description.abstractThe structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Rio Grande do Norte, Dept Bioquim, BR-59072970 Natal, RN, Brazil
dc.description.affiliationUniv Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN, Brazil
dc.description.affiliationUniv Liverpool, Dept Struct & Chem Biol, Liverpool L69 7ZB, Merseyside, England
dc.description.affiliationDiamond Light Source Ltd, Didcot OX11 0DE, Oxon, England
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipPPG.BIOQ (Programa de Pos-graduagao em Bioquimica/UFRN)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipLais
dc.format.extent372-378
dc.identifierhttp://dx.doi.org/10.1016/j.carbpol.2013.08.063
dc.identifier.citationCarbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014.
dc.identifier.doi10.1016/j.carbpol.2013.08.063
dc.identifier.fileWOS000329256600048.pdf
dc.identifier.issn0144-8617
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37305
dc.identifier.wosWOS:000329256600048
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofCarbohydrate Polymers
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectHeparinen
dc.subjectHeparan sulfateen
dc.subjectHemorrhagic activityen
dc.subjectAntithrombinen
dc.subjectAnticoagulanten
dc.subjectThermostabilizationen
dc.titleA non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potentialen
dc.typeinfo:eu-repo/semantics/article
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