Cimetidine Reduces Interleukin-6, Matrix Metalloproteinases-1 and-9 Immunoexpression in the Gingival Mucosa of Rat Molars With Induced Periodontal Disease

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dc.citation.issue1
dc.citation.volume88
dc.contributor.authorOliveira, Priscila Aparecida de [UNIFESP]
dc.contributor.authorPizzol Junior, Jose Paulo de [UNIFESP]
dc.contributor.authorLonghini, Renata [UNIFESP
dc.contributor.authorSasso-Cerri, Estela
dc.contributor.authorCerri, Paulo Sergio
dc.coverageChicago
dc.date.accessioned2020-07-31T12:46:46Z
dc.date.available2020-07-31T12:46:46Z
dc.date.issued2017
dc.description.abstractBackground: Histamine seems to act, via H-2 receptor, on inflammatory processes by stimulating interleukin (IL)-6 and matrix metalloproteinase (MMP) release. As cimetidine is an H-2 receptor antagonist, the authors hypothesize that this antiulcer drug reduces IL-6, MMP-1, andMMP-9immunoexpressioningingiva with inducedperiodontal disease (PD). Toconfirmapossible modulatory role of IL-6 on MMPs, the relationship between IL-6/MMP-1 and IL-6/MMP-9 immunoexpression was evaluated. Methods: Forty-six male rats were distributed into the cimetidine group (CimG: received daily intraperitoneal injections of 100 mg/kg of body weight of cimetidine) or the saline group (SG). PD was induced by cotton ligature around the maxillary left first molars (PDSG and PDCimG). The right molars were used as controls (SG and CimG). After 7, 15, 30, and 50 days, maxillary fragments were processed for paraffin embedding or for transmission electron microscopy. Tartrateresistant acid phosphatase (TRAP)-positive osteoclasts in the alveolar process surface and number of IL-6, MMP-1, and MMP-9-immunolabeled cells in the gingival mucosa were quantified. Statistical analyses were performed (P <= 0.05). Results: In PDSG and PDCimG, gingival mucosa exhibited few collagen fibers among numerous inflammatory cells. In PDCimG, the number of TRAP-positive osteoclasts and IL-6, MMP-1, andMMP-9-immunolabeled cells was significantly lower than in PDSG at all periods. A positive correlation between IL-6/MMP-1 and IL-6/MMP-9 was detected in PDSG and PDCimG. Conclusion: Cimetidine decreases bone loss through reduction of osteoclast number and induces reduction of IL-6, MMP-1, and MMP-9 immunoexpression, reinforcing the idea that the beneficial effect of cimetidine in PD may be due to reduction of IL-6 immunolabeling in the inflamed gingival mucosa.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Morphol & Genet, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Lab Histol & Embryol, Dept Morphol, Sch Dent, Araraquara, SP, Brazil
dc.description.affiliationUnifespDepartment of Morphology and Genetics, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2010/10391-9
dc.description.sponsorshipIDFAPESP: 2010/06912-3
dc.description.sponsorshipIDCNPq: 309533/2015-5
dc.format.extent100-111
dc.identifierhttp://dx.doi.org/10.1902/jop.2016.160132
dc.identifier.citationJournal Of Periodontology. Chicago, v. 88, n. 1, p. 100-111, 2017.
dc.identifier.doi10.1902/jop.2016.160132
dc.identifier.issn0022-3492
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56357
dc.identifier.wosWOS:000394506100015
dc.language.isoeng
dc.publisherAmer Acad Periodontology
dc.relation.ispartofJournal Of Periodontology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCimetidineen
dc.subjectgingivaen
dc.subjectimmunohistochemistryen
dc.subjectinterleukin-6en
dc.subjectosteoclasten
dc.subjectperiodontitisen
dc.titleCimetidine Reduces Interleukin-6, Matrix Metalloproteinases-1 and-9 Immunoexpression in the Gingival Mucosa of Rat Molars With Induced Periodontal Diseaseen
dc.typeinfo:eu-repo/semantics/article
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