Modulation of kinin B1 receptor expression by endogenous angiotensin II in hypertensive rats

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dc.contributor.authorFernandes, Liliarn
dc.contributor.authorCeravolo, Graziela S.
dc.contributor.authorFortes, Zuleica B.
dc.contributor.authorTostes, Rita
dc.contributor.authorSantos, Robson A. S.
dc.contributor.authorSantos, Jerusa A.
dc.contributor.authorMori, Marcelo A. S.
dc.contributor.authorPesquero, Joao Bosco
dc.contributor.authorCarvalho, Maria Helena C. de
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:29Z
dc.date.available2016-01-24T12:41:29Z
dc.date.issued2006-09-11
dc.description.abstractWe investigated the expression and localization of B I receptor in tissues of rats submitted to a renin-dependent model of hypertension (2K-IC), and analyzed the influence of endogenous Ang II in modulating the in vivo expression of these receptors. B1 mRNA levels in the heart, kidney and thoracic aorta were quantified by real time PCR, B1 receptor protein expression was assessed by immunchistochemistry, plasma Ang II levels were analyzed by radioirnmunoassay and the effects of ATI receptor blockade were determined after losartan treatment. 2K-IC rats presented a marked increase in Ang II levels when compared to sham-operated rats. in parallel, cardiac- (but not renal and aortic) B I mRNA levels were 15-fold higher in 2K-IC than in sham rats. in 2K-IC, B1 expression was detected in the endothelium of small cardiac arteries and in cardiomyocytes. Losartan completely reverted the increased B1 mRNA levels and significantly decreased the protein expression observed in 2K-IC rats, despite reducing, but not normalizing blood pressure. We conclude that in the 2K-IC rat, induction of cardiac B I receptor might be tightly linked to ATI receptor activation. These data suggest the existence of a new site of interaction between kinins and angiotensins, and might provide important contributions for a better understanding of the pathophysiology of hypertension. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 São Paulo, SP, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Dept Physiol & Biophys, Inst Biomed Sci, Belo Horizonte, MG, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent92-97
dc.identifierhttp://dx.doi.org/10.1016/j.regpep.2006.04.018
dc.identifier.citationRegulatory Peptides. Amsterdam: Elsevier B.V., v. 136, n. 1-3, p. 92-97, 2006.
dc.identifier.doi10.1016/j.regpep.2006.04.018
dc.identifier.issn0167-0115
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29155
dc.identifier.wosWOS:000241177200013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRegulatory Peptides
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectB1 receptoren
dc.subjectAT1 receptoren
dc.subject2 kidneys-1 clipen
dc.subjecthypertensionen
dc.subjectlosartanen
dc.titleModulation of kinin B1 receptor expression by endogenous angiotensin II in hypertensive ratsen
dc.typeinfo:eu-repo/semantics/article
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