Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential

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dc.contributor.authorFaloni, Ana Paula de Souza [UNIFESP]
dc.contributor.authorSchoenmaker, Ton
dc.contributor.authorAzari, Azin
dc.contributor.authorKatchburian, Eduardo [UNIFESP]
dc.contributor.authorCerri, Paulo Sergio [UNIFESP]
dc.contributor.authorVries, Teun J. de
dc.contributor.authorEverts, Vincent
dc.contributor.institutionUniv Amsterdam
dc.contributor.institutionVrije Univ Amsterdam
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Paulista UNESP
dc.date.accessioned2016-01-24T14:05:50Z
dc.date.available2016-01-24T14:05:50Z
dc.date.issued2011-01-01
dc.description.abstractOsteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. the expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. the jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. in conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow.en
dc.description.affiliationUniv Amsterdam, Dept Oral Cell Biol & Periodontol, Acad Ctr Dent Amsterdam ACTA, Res Inst Move, NL-1081 LA Amsterdam, Netherlands
dc.description.affiliationVrije Univ Amsterdam, NL-1081 LA Amsterdam, Netherlands
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Morphol, Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDCAPES: BEX:1174/08-8
dc.format.extent63-74
dc.identifierhttp://dx.doi.org/10.1007/s00223-010-9418-4
dc.identifier.citationCalcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011.
dc.identifier.doi10.1007/s00223-010-9418-4
dc.identifier.fileWOS000286201200009.pdf
dc.identifier.issn0171-967X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33179
dc.identifier.wosWOS:000286201200009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCalcified Tissue International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectJawen
dc.subjectLong boneen
dc.subjectOsteoclastogenesisen
dc.subjectOsteoclast precursoren
dc.subjectHeterogeneityen
dc.subjectMarrowen
dc.titleJaw and Long Bone Marrows Have a Different Osteoclastogenic Potentialen
dc.typeinfo:eu-repo/semantics/article
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