Molecular identification of Histoplasma capsulatum using rolling circle amplification

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dc.contributor.authorFuruie, Jason L.
dc.contributor.authorSun, Jiufeng
dc.contributor.authordo Nascimento, Mariana M. F.
dc.contributor.authorGomes, Renata R.
dc.contributor.authorWaculicz-Andrade, Caroline E.
dc.contributor.authorSessegolo, Gisele C.
dc.contributor.authorRodrigues, Anderson M. [UNIFESP]
dc.contributor.authorGalvao-Dias, Maria A.
dc.contributor.authorde Camargo, Zoilo P. [UNIFESP]
dc.contributor.authorQueiroz-Telles, Flavio
dc.contributor.authorNajafzadeh, Mohammad J.
dc.contributor.authorde Hoog, Sybren G.
dc.contributor.authorVicente, Vania A.
dc.date.accessioned2019-01-21T10:30:18Z
dc.date.available2019-01-21T10:30:18Z
dc.date.issued2016
dc.description.abstractHistoplasmosis is a systemic fungal disease that occurs worldwide, causing symptomatic infection mostly in immunocompromised hosts. Etiological agent is the dimorphic fungus, Histoplasma capsulatum, which occurs in soil contaminated with bird or bat droppings. Major limitation in recognition of H. capsulatum infections is the low awareness, since other diseases may have similar symptomatology. The molecular methods have gained importance because of unambiguous diagnostic ability and efficiency. The aim of this study was to develop and evaluate a padlock probe in view of rolling circle amplification (RCA) detection method which targets ITS (Internal Transcribed Spacer) rDNA of H. capsulatum enabling rapid and specific detection of the fungus in clinical samples. Two padlock probes were designed and one of these (HcPL2) allowed specific amplification of H. capsulatumDNA while no cross-reactivity was observed with fungi used as negative controls. This method proved to be effective for H. capsulatum specific identification and demonstrated to be faster than the traditional method of microbiological identification.en
dc.description.affiliationUniv Fed Parana, Bioproc Engn & Biotechnol Dept, Postgrad Program Bioproc Engn & Biotechnol, BR-81531900 Curitiba, Parana, Brazil
dc.description.affiliationUniv Fed Parana, Basic Pathol Dept, Postgrad Program Microbiol Parasitol & Pathol, BR-81531900 Curitiba, Parana, Brazil
dc.description.affiliationUniv Fed Parana, Clin Hosp, BR-81531900 Curitiba, Parana, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Cellular Biol Div, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationCtr Zoonosis Control, Sao Paulo, Brazil
dc.description.affiliationEcossistema Environm Consulting, Curitiba, Parana, Brazil
dc.description.affiliationGuangdong Prov Ctr Dis Control & Prevent, Guangdong Prov Inst Publ Hlth, Guangzhou, Guangdong, Peoples R China
dc.description.affiliationCBS KNAW Fungal Biodivers Ctr, NL-3508 AD Utrecht, Netherlands
dc.description.affiliationMashhad Univ Med Sci, Parasitol & Mycol Dept, Mashhad, Iran
dc.description.affiliationUnifespVicente, VA (reprint author), Univ Fed Parana, Bioproc Engn & Biotechnol Dept, Postgrad Program Bioproc Engn & Biotechnol, BR-81531900 Curitiba, Parana, Brazil.
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent12-19
dc.identifierhttp://dx.doi.org/10.1111/myc.12426
dc.identifier.citationMycoses. Hoboken, v. 59, n. 1, p. 12-19, 2016.
dc.identifier.doi10.1111/myc.12426
dc.identifier.issn0933-7407
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49692
dc.identifier.wosWOS:000367095200003
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofMycoses
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectHistoplasmosisen
dc.subjectHistoplasma capsulatumen
dc.subjectdiagnosisen
dc.subjectpadlock probeen
dc.subjectRCARapid Identificationen
dc.subjectGenetic Diversityen
dc.subjectSpecies Complexen
dc.subjectPcren
dc.subjectSequenceen
dc.subjectRegionsen
dc.subjectPolymorphismen
dc.subjectBrazilen
dc.subjectAssayen
dc.subjectDnaen
dc.titleMolecular identification of Histoplasma capsulatum using rolling circle amplificationen
dc.typeinfo:eu-repo/semantics/article
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