Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine

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dc.citation.issue2
dc.citation.volume32
dc.contributor.authorSiqueira das Neves, Lais Mara
dc.contributor.authorMarcondes Ferreira Leite, Gabriella de Paula
dc.contributor.authorMarcolino, Alexandre Marcio
dc.contributor.authorPinfildi, Carlos Eduardo [UNIFESP]
dc.contributor.authorGarcia, Sergio Britto
dc.contributor.authorde Araujo, Joao Eduardo
dc.contributor.authorde Oliveira Guirro, Elaine Caldeira
dc.coverageLondon
dc.date.accessioned2020-07-17T14:03:09Z
dc.date.available2020-07-17T14:03:09Z
dc.date.issued2017
dc.description.abstractThe aim of this study was to investigate the effect of laser photobiomodulation (PBM) on the viability of the transverse rectus abdominis musculocutaneous (TRAM) flap in rats subjected to the action of nicotine. We evaluated 60 albino Wistar rats, divided into six groups of ten animals. Group 1 (saline) underwent the surgical technique to obtain a TRAM flapen
dc.description.abstractgroup 2 (laser 830 nm) underwent the surgical technique and was irradiated with a laser 830 nmen
dc.description.abstractgroup 3 (laser 660 nm) underwent the surgical technique and was irradiated with a laser 660 nmen
dc.description.abstractgroup 4 was treated with nicotine subcutaneously (2 mg/kg/2x/day/4 weeks) and underwent surgeryen
dc.description.abstractgroup 5 (nicotine + laser 830 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 830 nmen
dc.description.abstractgroup 6 (nicotine + laser 660 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 660 nm. The application of PBM occurred immediately after surgery and on the two following days. The percentage of necrosis was assessed using the AxioVisionA (R) software. The number of mast cells (toluidine blue staining) was evaluated, and immunohistochemistry was performed to detect vascular endothelial growth factor expression (anti-VEGF-A), fibroblasts (anti-basic FGF), and neoformed vessels (anti-CD34). PBM with a wavelength of 830 nm increased the viability of the TRAM flap, with a smaller area of necrosis, increased number of mast cells, and higher expression of VEGF and CD34. PBM increases the viability of musculocutaneous flaps treated with to nicotine.en
dc.description.affiliationUniv Sao Paulo FMRP USP, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed Santa Catarina, Postgrad Program Rehabil Sci, UFSC SC, Campus Ararangua, Florianopolis, SC, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Postgrad Program Interdisciplinary Hlth Sci, UNIFESP SP, Santos Campus, Sao Paulo, Brazil
dc.description.affiliationFMRP USP, Postgrad Program Pathol, Ribeirao Preto Med Sch, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Postgrad Program Interdisciplinary Hlth Sci, UNIFESP SP, Santos Campus, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFoundation of Sao Paulo State Research (FAPESP)
dc.description.sponsorshipIDFAPESP: 2013/07602-6
dc.description.sponsorshipIDFAPESP: 2013/07732-7
dc.format.extent335-341
dc.identifierhttp://dx.doi.org/10.1007/s10103-016-2118-1
dc.identifier.citationLasers In Medical Science. London, v. 32, n. 2, p. 335-341, 2017.
dc.identifier.doi10.1007/s10103-016-2118-1
dc.identifier.issn0268-8921
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55189
dc.identifier.wosWOS:000394154600010
dc.language.isoeng
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers In Medical Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectLow-level laser therapyen
dc.subjectPhototherapyen
dc.subjectDosimetryen
dc.subjectPhysical therapyen
dc.subjectVascular endothelial growth factoren
dc.subjectFibroblast growth factoren
dc.subjectNicotineen
dc.titleLaser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotineen
dc.typeinfo:eu-repo/semantics/article
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