Repeated stress reduces mucociliary clearance in animals with chronic allergic airway inflammation

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dc.contributor.authorAlmeida-Reis, Rafael
dc.contributor.authorToledo, Alessandra Choqueta
dc.contributor.authorReis, Fabiana Gomes
dc.contributor.authorMarques, Ricardo Henrique
dc.contributor.authorPrado, Carla Máximo [UNIFESP]
dc.contributor.authorDolhnikoff, Marisa
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorLeick-Maldonado, Edna Aparecida
dc.contributor.authorTiberio, Iolanda de Fátima Lopes Calvo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionLusiada Fac Med Sci
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:20Z
dc.date.available2016-01-24T14:05:20Z
dc.date.issued2010-08-31
dc.description.abstractWe evaluated if repeated stress modulates mucociliary clearance and inflammatory responses in airways of guinea pigs (GP) with chronic inflammation. the GP received seven exposures of ovalbumin or saline 0.9%. After 4th inhalation, animals were submitted to repeated forced swim stressor protocol (5x/week/2 weeks). After 7th inhalation, GP were anesthetized. We measured transepithelial potential difference, ciliary beat frequency, mucociliary transport, contact angle, cough transportability and serum cortisol levels. Lungs and adrenals were removed, weighed and analyzed by morphometry. Ovalbumin-exposed animals submitted to repeated stress had a reduction in mucociliary transport, and an increase on serum cortisol, adrenals weight, mucus wettability and adhesivity, positive acid mucus area and IL-4 positive cells in airway compared to non-stressed ovalbumin-exposed animals (p < 0.05). There were no effects on eosinophilic recruitment and IL-13 positive cells. Repeated stress reduces mucociliary clearance due to mucus theological-property alterations, increasing acid mucus and its wettability and adhesivity. These effects seem to be associated with IL-4 activation. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Dept Clin Med, Fac Med, Sch Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Pathol, Sch Med, BR-01246903 São Paulo, Brazil
dc.description.affiliationLusiada Fac Med Sci, Dept Physiol, Santos, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipExperimental Therapeutics Laboratory
dc.description.sponsorshipIDExperimental Therapeutics Laboratory: LIM-20
dc.format.extent79-85
dc.identifierhttps://dx.doi.org/10.1016/j.resp.2010.06.011
dc.identifier.citationRespiratory Physiology & Neurobiology. Amsterdam: Elsevier B.V., v. 173, n. 1, p. 79-85, 2010.
dc.identifier.doi10.1016/j.resp.2010.06.011
dc.identifier.issn1569-9048
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/32829
dc.identifier.wosWOS:000282025500012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRespiratory Physiology & Neurobiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectStressen
dc.subjectExperimental model of chronic airwayen
dc.subjectinflammationen
dc.subjectMucociliary propertiesen
dc.subjectLung inflammationen
dc.titleRepeated stress reduces mucociliary clearance in animals with chronic allergic airway inflammationen
dc.typeinfo:eu-repo/semantics/article
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