Metoclopramide-induced hyperprolactinaemia caused marked decline in pinopodes and pregnancy rates in mice

Página do item simplificado
dc.contributor.authorPanzan, Michele Quarante [UNIFESP]
dc.contributor.authorSoares Junior, José Maria [UNIFESP]
dc.contributor.authorMotta, Eduardo Leme Alves da [UNIFESP]
dc.contributor.authorHaapalainen, Edna Freymuller [UNIFESP]
dc.contributor.authorJesus Simoes, Manuel de [UNIFESP]
dc.contributor.authorBaptista, Heloisa Allegro [UNIFESP]
dc.contributor.authorHaidar, Mauro Abi [UNIFESP]
dc.contributor.authorBaracat, Edmund Chada [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:29Z
dc.date.available2016-01-24T12:41:29Z
dc.date.issued2006-10-01
dc.description.abstractBACKGROUND: the impact of hyperprolactinaemia on endometrial function, along with embryo implantation, has been the subject of discussion. This article examines whether experimental hyperprolactinaemia can affect mouse ovarian function, endometrial pinopodes and embryo implantation. METHODS: for pinopode analysis, 60 female mice were randomly divided into two groups: control (vehicle) and experimental [metoclopramide (MCP) 200 mu g per day]. Injections were given subcutaneously for 50 days, and then, normally cycling females were housed with male mice for copulation during proestrus. the animals were killed on the fifth day following coitus when the antimesometrium portions of the uterine horns were removed for endometrial analysis. Blood was collected for prolactin (PRL) determination. in the second experiment, 60 female mice were used to evaluate the ovarian function by measuring estrogen and progesterone levels and counting luteal bodies and oocytes in the oviduct and uterus during estrus. RESULTS: the highest pregnancy rates and the largest population of pinopodes were both found in the vehicle group (P < 0.01). Estrogen and progesterone levels in MCP-treated mice were lower than those in control mice (P < 0.05). Also, the number of implantations was significantly lower in the MCP-treated group compared with the vehicle group after embryo transfer (P < 0.001). CONCLUSION: PRL seems to have suppressive effects on ovarian function and the number of pinopodes; conceivably, hyperprolactinaemia has a negative effect on mouse embryo implantation.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, CEDEME, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, CEDEME, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent2514-2520
dc.identifierhttp://dx.doi.org/10.1093/humrep/del093
dc.identifier.citationHuman Reproduction. Oxford: Oxford Univ Press, v. 21, n. 10, p. 2514-2520, 2006.
dc.identifier.doi10.1093/humrep/del093
dc.identifier.issn0268-1161
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29164
dc.identifier.wosWOS:000240928600010
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofHuman Reproduction
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectembryo implantationen
dc.subjecthyperprolactinaemiaen
dc.subjectmetoclopramideen
dc.subjectmiceen
dc.subjectpinopodesen
dc.titleMetoclopramide-induced hyperprolactinaemia caused marked decline in pinopodes and pregnancy rates in miceen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos