Impedance sensing of DNA hybridization onto nanostructured phthalocyanine-modified electrodes

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dc.citation.volume221
dc.contributor.authorManzanares-Palenzuela, C. L.
dc.contributor.authorFernandes, Edson Giuliani Ramos [UNIFESP]
dc.contributor.authorLobo-Castanon, M. J.
dc.contributor.authorLopez-Ruiz, B.
dc.contributor.authorZucolotto, V.
dc.coverageOxford
dc.date.accessioned2020-07-31T12:47:03Z
dc.date.available2020-07-31T12:47:03Z
dc.date.issued2016
dc.description.abstractDNA detection is still undergoing major innovations in pursuit of low-cost and simple approaches for decentralized applications. Label-free sensing of DNA hybridization via impedance measurements is a popular strategy to fulfil the goals of cost-efficiency and simplicity. Several materials are often reported for electrode modification to improve the sensitivity of impedance-based sensors. Herein we evaluate the electronic properties of copper phthalocyanine tetrasulfonate (CuPcTs) in Layer-by-Layer (LbL) films for impedimetric sensing of DNA hybridization using silanized Fluorine-doped Tin Oxide (FTO) electrodes. 1 to 5 bilayers were prepared by alternate immersion of the substrate in CuPcTs and poly(allylamine hydrochloride) (PAH). DNA probe immobilization was carried out electrostatically onto the last PAH layer, followed by hybridization with the target sequence leading to the formation of a partial double stranded (pds) structure onto the films. Impedance decreased after hybridization proportionally with the concentration of the target sequence at picomolar levels. Not only are these findings useful as a potential biosensing strategy, but also leave an open question about the electronic and synergistic properties of DNA interacting with different materials and surfaces. (C) 2016 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566970 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencia & Tecnol, Ave Cesare Mansueto Giulio Lattes 1201, BR-12247014 Sao Jose Dos Campos, Brazil
dc.description.affiliationUniv Oviedo, Fac Quim, Calle Juan Claveria 8, Oviedo 33006, Spain
dc.description.affiliationUniv Complutense Madrid, Fac Farm, Plaza Ramon y Cajal S-N, E-28040 Madrid, Spain
dc.description.affiliationUnifespInstituto de Ciência e Tecnologia, Universidade Federal de São Paulo, Avenida Cesare Mansueto Giulio Lattes 1201, 12247-014 São José dos Campos, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMarie Curie Actions FP7-PEOPLE-IRSES
dc.description.sponsorshipUCM
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDMarie Curie Actions FP7-PEOPLE-IRSES: 612545
dc.description.sponsorshipIDUCM: BE43/11
dc.format.extent86-95
dc.identifierhttp://dx.doi.org/10.1016/j.electacta.2016.10.140
dc.identifier.citationElectrochimica Acta. Oxford, v. 221, p. 86-95, 2016.
dc.identifier.doi10.1016/j.electacta.2016.10.140
dc.identifier.issn0013-4686
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56554
dc.identifier.wosWOS:000390746600012
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofElectrochimica Acta
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCopper phthalocyanine tetrasulfonateen
dc.subjectCuPcTsen
dc.subjectDNA sensingen
dc.subjectElectrochemical Impedance Spectroscopyen
dc.subjectAtomic Force Microscopyen
dc.subjectFTO electrodesen
dc.titleImpedance sensing of DNA hybridization onto nanostructured phthalocyanine-modified electrodesen
dc.typeinfo:eu-repo/semantics/article
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