Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome

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dc.contributor.authorAitken, Elizabeth H.
dc.contributor.authorNegri, Elnara M.
dc.contributor.authorBarboza, Renato [UNIFESP]
dc.contributor.authorLima, Maria Ri
dc.contributor.authorAlvarez, Jose M.
dc.contributor.authorMarinho, Claudio R. F.
dc.contributor.authorCaldini, Elia G.
dc.contributor.authorEpiphanio, Sabrina
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:37:27Z
dc.date.available2016-01-24T14:37:27Z
dc.date.issued2014-06-13
dc.description.abstractBackground: the mechanisms through which infection with Plasmodium spp. result in lung disease are largely unknown. Recently a number of mouse models have been developed to research malaria-associated lung injury but no detailed ultrastructure studies of the disease in its terminal stages in a murine model have yet been published. the goal was to perform an ultrastructural analysis of the lungs of mice that died with malaria-associated acute lung injury/acute respiratory distress syndrome to better determine the relevancy of the murine models and investigate the mechanism of disease.Methods: DBA/2 mice were infected with Plasmodium berghei strain ANKA. Mice had their lungs removed immediately after death, processed using standard methods and viewed by transmission electron microscopy (TEM).Results: Infected red blood cell: endothelium contact, swollen endothelium with distended cytoplasmic extensions and thickening of endothelium basement membrane were observed. Septa were thick and filled with congested capillaries and leukocytes and the alveolar spaces contained blood cells, oedema and cell debris.Conclusion: Results show that the lung ultrastructure of P. berghei ANKA-infected mice has similar features to what has been described in post-mortem TEM studies of lungs from individuals infected with Plasmodium falciparum. These data support the use of murine models to study malaria-associated acute lung injury.en
dc.description.affiliationUniv São Paulo, Dept Immunol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Lab Med Invest 59, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Phys & Earth Sci, Diadema, Brazil
dc.description.affiliationUniv São Paulo, Dept Parasitol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Phys & Earth Sci, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2009/53256-7
dc.description.sponsorshipIDFAPESP: 2009/53889-0
dc.description.sponsorshipIDCNPq: 306668/2012-2
dc.description.sponsorshipIDFAPESP: 2011/195252-0
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1186/1475-2875-13-230
dc.identifier.citationMalaria Journal. London: Biomed Central Ltd, v. 13, 10 p., 2014.
dc.identifier.doi10.1186/1475-2875-13-230
dc.identifier.fileWOS000338889000001.pdf
dc.identifier.issn1475-2875
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37872
dc.identifier.wosWOS:000338889000001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofMalaria Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMalariaen
dc.subjectPlasmodiumen
dc.subjectAcute lung injury (ALI)en
dc.subjectAcute respiratory distress syndrome (ARDS)en
dc.subjectTransmission electron microscopyen
dc.subjectMurine modelen
dc.titleUltrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndromeen
dc.typeinfo:eu-repo/semantics/article
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