Aerobic exercise inhibits acute lung injury: from mouse to human evidence Exercise reduced lung injury markers in mouse and in cells
dc.citation.volume | 24 | |
dc.contributor.author | Rigonato-Oliveira, Nicole Cristine | |
dc.contributor.author | MacKenzie, BreAnne | |
dc.contributor.author | Bachi, Andre Luis Lacerda | |
dc.contributor.author | Oliveira-Junior, Manoel Carneiro | |
dc.contributor.author | Dias, Alana dos Santos | |
dc.contributor.author | Rodrigues Brandao-Rangel, Maysa Alves [UNIFESP] | |
dc.contributor.author | Delle, Humberto | |
dc.contributor.author | Costa-Guimaraes, Tamara | |
dc.contributor.author | Damaceno-Rodrigues, Nilsa Regina | |
dc.contributor.author | Dulley, Larissa Hilario | |
dc.contributor.author | Benetti, Marcela Anhesini | |
dc.contributor.author | Malfitano, Christiane | |
dc.contributor.author | De Angelis, Kátia [UNIFESP] | |
dc.contributor.author | Albertini, Regiane [UNIFESP] | |
dc.contributor.author | Oliveira, Ana Paula Ligeiro | |
dc.contributor.author | Abbasi, Asghar | |
dc.contributor.author | Northoff, Hinnak | |
dc.contributor.author | Vieira, Rodolfo de Paula [UNIFESP] | |
dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
dc.coverage | Greven | |
dc.date.accessioned | 2020-07-02T18:52:00Z | |
dc.date.available | 2020-07-02T18:52:00Z | |
dc.date.issued | 2018 | |
dc.description.abstract | Acute respiratory distress syndrome (ARDS) is defined as hypoxemic respiratory failure with intense pulmonary inflammation, involving hyperactivation of endothelial cells and neutrophils. Given the anti-inflammatory effects of aerobic exercise (AE), this study investigated whether AE performed daily for 5 weeks would inhibit extra-pulmonary LPS-induced ARDS. C57Bl/6 mice were distributed into Control, Exercise, LPS and Exercise+ LPS groups. AE was performed on a treadmill for 5x/week for four weeks before LPS administration. 24hours after the final AE physical test, animals received 100ug of LPS intra-peritoneally. In addition, whole blood cell culture, neutrophils and human endothelial cells were pre-incubated with IL-10, an anti-inflammatory cytokine induced by exercise. AE reduced total protein levels (p<0.01) and neutrophil accumulation in bronchoalveolar lavage (BAL) (p<0.01) and lung parenchyma (p<0.01). AE reduced BAL inflammatory cytokines IL-1 beta, IL-6 and GM-CSF (p<0.001), CXCL1/KC, IL-17, TNF-alpha and IGF-1 (p<0.01). Systemically, AE reduced IL-1 beta, IL-6 and IFN-gamma (p<0.001), CXCL1/KC (p<0.01) and TNF-alpha (p<0.05). AE increased IL-10 levels in serum (p<0.001) and BAL (p<0.001). Furthermore, AE increased superoxide dismutase SOD (p<0.01) and decreased superoxide anion accumulation in the lungs (p<0.01). Lastly, pre-incubation with IL-10 significantly reduced LPS-induced activation of whole blood cells, neutrophils and HUVECs, as observed by reduced production of IL-1 beta, IL-6, IL-8 and TNF-alpha. Our data suggest that AE inhibited LPS-induced lung inflammation by attenuating inflammatory cytokines and oxidative stress markers in mice and human cell culture via enhanced IL-10 production. | en |
dc.description.affiliation | Nove Julho Univ, Sao Paulo, SP, Brazil | |
dc.description.affiliation | Brazilian Inst Teaching & Res Pulm & Exercise Imm, Sao Jose Dos Campos, SP, Brazil | |
dc.description.affiliation | Fed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Santos, SP, Brazil | |
dc.description.affiliation | Univ Brasil, Sao Paulo, SP, Brazil | |
dc.description.affiliation | Univ Sao Paulo, Sch Med, Dept Pathol LIM 59, Sao Paulo, SP, Brazil | |
dc.description.affiliation | Univ Fed Lavras UFLA, Sci Dept Hlth, Lavras, MG, Brazil | |
dc.description.affiliation | Fed Univ Sao Paulo UNIFESP, Campus Sao Paulo, Sao Paulo, SP, Brazil | |
dc.description.affiliation | Harbor UCLA Med Ctr, Div Resp & Crit Care Physiol & Med, Los Angeles Biomed Res Inst, Torrance, CA 90509 USA | |
dc.description.affiliation | Univ Tubingen, Inst Clin & Expt Transfus Med IKET, Tubingen, Germany | |
dc.description.affiliationUnifesp | Fed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Santos, SP, Brazil | |
dc.description.affiliationUnifesp | Fed Univ Sao Paulo UNIFESP, Campus Sao Paulo, Sao Paulo, SP, Brazil | |
dc.description.source | Web of Science | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | pt |
dc.description.sponsorshipID | FAPESP: 2012/15165-2 | pt |
dc.description.sponsorshipID | CNPq: 311335-2015-2 | pt |
dc.description.sponsorshipID | CAPES: 12804/13-4 | pt |
dc.description.sponsorshipID | CAPES: 1303/13-9 | pt |
dc.description.sponsorshipID | FAPESP: 2014/23196-0 | pt |
dc.description.sponsorshipID | FAPESP: 2012/14604-8 | pt |
dc.description.sponsorshipID | FAPESP: 2012/25435-7 | pt |
dc.description.sponsorshipID | FAPESP: 2012/24880-7 | pt |
dc.description.sponsorshipID | FAPESP: 2013/24076-6 | pt |
dc.format.extent | 48-56 | |
dc.identifier | https://eir-isei.de/2018/eir-2018-036-article.pdf | |
dc.identifier.citation | Exercise Immunology Review. Greven, v. 24, p. 48-56, 2018. | |
dc.identifier.file | WOS000428135000005.pdf | |
dc.identifier.issn | 1077-5552 | |
dc.identifier.uri | https://repositorio.unifesp.br/handle/11600/53814 | |
dc.identifier.wos | WOS:000428135000005 | |
dc.language.iso | eng | |
dc.publisher | W W F Verlagsgesellschaft Gmbh | |
dc.relation.ispartof | Exercise Immunology Review | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Exercise immunology | en |
dc.subject | Lung inflammation | en |
dc.subject | Immune response | en |
dc.subject | Acute lung injury | en |
dc.subject | Cytokines | en |
dc.title | Aerobic exercise inhibits acute lung injury: from mouse to human evidence Exercise reduced lung injury markers in mouse and in cells | en |
dc.type | info:eu-repo/semantics/article |
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