Effects of customized bicarbonate buffered solutions for continuous renal replacement therapies on polymorphonuclear leukocytes function and viability

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dc.contributor.authorMaccariello, E.
dc.contributor.authorDalboni, Maria Aparecida [UNIFESP]
dc.contributor.authorDraibe, S. A.
dc.contributor.authorRocha, E.
dc.contributor.authorSantos, OFP dos
dc.contributor.authorSantos, B.
dc.contributor.authorCendoroglo, M.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionAlbert Einstein Jewish Hosp
dc.date.accessioned2016-01-24T12:37:12Z
dc.date.available2016-01-24T12:37:12Z
dc.date.issued2004-06-01
dc.description.abstractIt has previously been shown that the mixture of bicarbonate and calcium in the solutions used for continuous renal replacement therapy led to crystalization and significant changes in calcium concentration and pH. the aim of this study was to investigate the impact of bicarbonate/calcium and lactate/calcium solutions for Continuous Renal Replacement Therapies (CRRT) on the viability and function of polymorphonuclear cells (PMN). We tested four customized bicarbonate buffered solutions: single bag (bicarbonate and calcium mixed 24 h before testing), double bag (mixed immediately before testing), filtered single bag and double bag solutions, and a commercial lactate buffered solution. Blood from 6 volunteers was incubated with the solutions for 30 min followed by PMN isolation. After overnight incubation, viability, phagocytosis, and peroxide production by PMN were determined by flow cytometry. There was no difference between the test solutions with respect to PMN viability and function. Therefore, the presence of microcrystals and the consequent changes in electrolyte concentrations do not seem to impair PMN function.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUniv Fed Fluminense, Div Nephrol, Rio de Janeiro, Brazil
dc.description.affiliationFed Univ Rio de Janeiro, HUCFF, Multidisciplinary Lab, Rio de Janeiro, Brazil
dc.description.affiliationAlbert Einstein Jewish Hosp, Dialysis Unit, São Paulo, Brazil
dc.description.affiliationAlbert Einstein Jewish Hosp, Intens Care Unit, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Div Nephrol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent571-576
dc.identifierhttp://dx.doi.org/10.1111/j.1525-1594.2004.07284.x
dc.identifier.citationArtificial Organs. Malden: Blackwell Publishing Inc, v. 28, n. 6, p. 571-576, 2004.
dc.identifier.doi10.1111/j.1525-1594.2004.07284.x
dc.identifier.issn0160-564X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27789
dc.identifier.wosWOS:000221532200009
dc.language.isoeng
dc.publisherBlackwell Publishing Inc
dc.relation.ispartofArtificial Organs
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectdialysisen
dc.subjecthemofiltrationen
dc.subjectsolutionsen
dc.subjectpolymorphonuclear cellsen
dc.subjectviabilityen
dc.subjectfunctionen
dc.titleEffects of customized bicarbonate buffered solutions for continuous renal replacement therapies on polymorphonuclear leukocytes function and viabilityen
dc.typeinfo:eu-repo/semantics/article
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