The role of growth factors on renal tubular cells submitted to hypoxia and deprived of glucose

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dc.contributor.authorDurao, M. D.
dc.contributor.authorRazvickas, C. V.
dc.contributor.authorGoncalves, EAP
dc.contributor.authorOkano, I. R.
dc.contributor.authorCamargo, SMR
dc.contributor.authorMonte, JCM
dc.contributor.authorSantos, OFP dos
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:40Z
dc.date.available2016-01-24T12:33:40Z
dc.date.issued2003-01-01
dc.description.abstractBackground. in acute renal failure (ARF) renal tubular cell death and detachment can be induced by necrotic and apoptotic mechanisms. Several studies have demonstrated some benefits of the use of growth factors in experimental models of ARF. Methods. MDCK cells were cultured in a glucose-free medium for 24 h and were submitted to hypoxia (pO(2) around 35 mmHg) for additional 24 h. To evaluate the possible protective role of growth factors, EGF, IGF-I or HGF were added to the medium (20 ng mL). LDH release, viability (acridine orange and ethidium bromide dyes) and quantification of apoptotic cells (Hoechst 33342 dye fluorescence) were determined. Results. in the injury group, an increase on LDH release (60% vs. 3%) and on number of apoptotic cells (22% vs. 0.2%) which was associated with a reduced cell viability (61% vs. 94%) when compared with controls. Only HGF, not EGF or IGF-I, was able to protect cells from injury. HGF caused a significant reduction on LDH release (30%) and on number of apoptotic cells (5%), with an increase on viability cellular (79%). Conclusions. HGF decreases cell death on MDCK cells after hypoxic-induced injury, probably acting in both necrotic and apoptotic mechanisms.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, Escola Paulista Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent341-353
dc.identifierhttp://dx.doi.org/10.1081/JDI-120021149
dc.identifier.citationRenal Failure. New York: Marcel Dekker Inc, v. 25, n. 3, p. 341-353, 2003.
dc.identifier.doi10.1081/JDI-120021149
dc.identifier.issn0886-022X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27106
dc.identifier.wosWOS:000183309000002
dc.language.isoeng
dc.publisherMarcel Dekker Inc
dc.relation.ispartofRenal Failure
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectARFen
dc.subjectgrowth factorsen
dc.subjectapoptosisen
dc.subjectrenal tubular cellsen
dc.subjecthypoxiaen
dc.titleThe role of growth factors on renal tubular cells submitted to hypoxia and deprived of glucoseen
dc.typeinfo:eu-repo/semantics/article
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