Effects of Low-Level Laser Therapy on Collagen Expression and Neutrophil Infiltrate in 5-Fluorouracil-Induced Oral Mucositis in Hamsters

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dc.contributor.authorFontana Lopes, Nilza Nelly [UNIFESP]
dc.contributor.authorPlapler, Helio [UNIFESP]
dc.contributor.authorLalla, Rajesh V.
dc.contributor.authorChavantes, Maria Cristina
dc.contributor.authorYoshimura, Elisabeth Mateus
dc.contributor.authorBastos da Silva, Marco Antonio [UNIFESP]
dc.contributor.authorSeixas Alves, Maria Teresa [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Connecticut
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:05:15Z
dc.date.available2016-01-24T14:05:15Z
dc.date.issued2010-08-01
dc.description.abstractBackground and Objectives: Several studies have suggested that low-level laser therapy (LLLT) can ameliorate oral mucositis, however, the mechanisms involved are not well understood. the aim of this study was to investigate the mechanisms of action of LLLT on chemotherapy-induced oral mucositis, as related to effects on collagen expression and inflammationMaterials and Methods: A hamster cheek pouch model of oral mucositis was used with all animals receiving intraperitoneal 5-fluorouracil, followed by surface irritation. Animals were randomly allocated into three groups, and treated with an InGaAIP diode laser at a wavelength of 660 nm and output power of 35 or 100 mW laser, or no laser Clinical severity of mucositis was assessed at four time-points by a blinded examiner Buccal pouch tissue was harvested from a subgroup of animals in each group at four time-points. Collagen was qualitatively and quantitatively evaluated after picrosinus staining. the density of the neutrophil infiltrate was also scoredResults: Peak clinical severity of mucositis was reduced in the 35 mW laser group as compared to the 100 mW and control groups the reduced peak clinical severity of mucositis in the 35 mW laser group was accompanied by a decrease in the number of neutrophils and an increase in the proportion of mature collagen as compared to the other two groups. the total quantity of collagen was significantly higher in the control (no laser) group at the day 11 time-point, as compared to the 35 mW laser group, consistent with a more prolonged inflammatory response in the control group.Conclusion: This study supports two mechanisms of action for LLLT in reducing mucositis severity. the increase in collagen organization in response to the 35 mW laser indicates that LLLT promotes wound healing in addition, LLLT also appears to have an anti-inflammatory effect, as evidenced by the reduction in neutrophil infiltrate Lasers Surg Med 42 546-552, 2010. (C) 2010 Wiley-Liss, Inc.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Expt Surg, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Connecticut, Ctr Hlth, Sect Oral Med, Farmington, CT 06030 USA
dc.description.affiliationUniv São Paulo, Sch Med, Med Laser Ctr, INCOR HC, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Phys, Dept Nucl Phys, BR-05315970 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Expt Surg, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent546-552
dc.identifierhttp://dx.doi.org/10.1002/lsm.20920
dc.identifier.citationLasers in Surgery and Medicine. Hoboken: Wiley-liss, v. 42, n. 6, p. 546-552, 2010.
dc.identifier.doi10.1002/lsm.20920
dc.identifier.issn0196-8092
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32761
dc.identifier.wosWOS:000281009400013
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofLasers in Surgery and Medicine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectchemotherapyen
dc.subjectcollagenen
dc.subjectinflammationen
dc.subjectlaseren
dc.subjectmucositisen
dc.subjectneutrophilen
dc.subjectstomatitisen
dc.titleEffects of Low-Level Laser Therapy on Collagen Expression and Neutrophil Infiltrate in 5-Fluorouracil-Induced Oral Mucositis in Hamstersen
dc.typeinfo:eu-repo/semantics/article
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