Differential Shh, Bmp and Wnt gene expressions during craniofacial development in mice

Página do item simplificado
dc.contributor.authorSilva Paiva, Katiucia Batista
dc.contributor.authorSilva-Valenzuela, Maria das Gracas
dc.contributor.authorGomes Massironi, Silvia Maria
dc.contributor.authorKo, Gui Mi [UNIFESP]
dc.contributor.authorSiqueira, Filipe Modolo
dc.contributor.authorNunes, Fabio Daumas
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.date.accessioned2016-01-24T13:59:05Z
dc.date.available2016-01-24T13:59:05Z
dc.date.issued2010-01-01
dc.description.abstractIn this study, Bmp-4, Wnt-5a and Shh gene expressions were compared during early craniofacial development in mice by comparative non-isotopic in situ hybridization. Wild-type C57BL/6J mice were studied at various stages of embryonic development (from 8.5- to 13.5-day-old embryos - E8.5-13.5). During early odontogenesis, transcripts for Bmp-4, Shh and Wnt-5a were co-localised at the tooth initiation stage. At E8.5, Shh mRNA expression was restricted to diencephalon and pharyngeal endoderm. Before maxillae and mandible ossification, Bmp-4 and Wnt-5a signals were detected in the mesenchymal cells and around Meckel's cartilage. During palatogenesis, Shh was expressed only in the epithelium and Wnt-5a only in the mesenchyme of the elevating palatal shelves. During tongue development, Shh expression was found in mesenchyme, probably contributing to tongue miogenesis, while Wnt-5a signal was in the epithelium, possibly during placode development and papillae formation. Taken together, these findings suggest that Bmp-4, Shh and Wnt-5a gene expressions may act together on the epithelial mesenchymal interactions occurring in several aspects of the early mouse craniofacial development, such as odontogenesis, neuronal development, maxillae and mandible ossification, palatogenesis and tongue formation. (C) 2009 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniv São Paulo, Dept Oral Pathol, Sch Dent, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Immunol, Inst Biomed Sci, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Dev Anim Models, São Paulo, Brazil
dc.description.affiliationUniv Fed Santa Catarina, Ctr Hlth Sci, Dept Pathol, BR-88040900 Florianopolis, SC, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Dev Anim Models, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 1997/13228-5
dc.format.extent508-517
dc.identifierhttp://dx.doi.org/10.1016/j.acthis.2009.05.007
dc.identifier.citationActa Histochemica. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 112, n. 5, p. 508-517, 2010.
dc.identifier.doi10.1016/j.acthis.2009.05.007
dc.identifier.issn0065-1281
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32083
dc.identifier.wosWOS:000282620700011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofActa Histochemica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBMP-4en
dc.subjectSHHen
dc.subjectWnt-5aen
dc.subjectCraniofacial developmenten
dc.subjectIn situ hybridizationen
dc.titleDifferential Shh, Bmp and Wnt gene expressions during craniofacial development in miceen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos