Sprouting of mossy fibers and the vacating of postsynaptic targets in the inner molecular layer of the dentate gyrus

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dc.contributor.authorLongo, B.
dc.contributor.authorCovolan, Luciene [UNIFESP]
dc.contributor.authorChadi, G.
dc.contributor.authorMello, LEAM
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUNIBAN
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:33:49Z
dc.date.available2016-01-24T12:33:49Z
dc.date.issued2003-05-01
dc.description.abstractAberrant mossy fiber sprouting, which presumably results from hilar mossy cell death after status epilepticus (SE), is a frequently studied feature of temporal lobe epilepsy. Although mossy fiber sprouting can be suppressed by the protein synthesis inhibitor cycloheximide, spontaneous seizures remain unaltered. We have investigated the mechanisms underlying the ability of cycloheximide to block SE-induced mossy fiber sprouting in the inner molecular layer of dentate gyrus (IML). Pilocarpine-induced SE in the presence of cycloheximide resulted in a reduced number of injured hilar cells compared to rats not pretreated with cycloheximide. Presumed mossy cells, identified by calcitonin gene related peptide (CGRP) immunohistochemistry, were not significantly reduced in either group 60 days after SE. Whereas controls had a strong band of CGRP-positive fibers (putative mossy cell axons) and no neo-Timm stained fibers in the IML, pilocarpine-treated rats had no CGRP fibers and strong neo-Timm staining. Cycloheximide-pilocarpine-treated animals, in contrast, had CGRP and neo-Timm staining similar to controls. Cycloheximide might protect hilar CGRP-positive cells during SE. and, by allowing those cells to retain their normal axonal projection, prevent mossy fiber sprouting. the recently suggested irritable mossy cell hypothesis relies on the survival of mossy cells for network hyperexcitability. We hypothesized that CGRP may be a marker for a subpopulation of relatively resistant mossy cells in rats, which, if they survive injury, may become irritable and contribute to hyperexcitability. We suggest that cycloheximide prevents SE-induced mossy fiber sprouting by preventing the loss of hilar CGRP-positive cells (putative mossy cells). (C) 2003 Elsevier Science (USA). All rights reserved.en
dc.description.affiliationUNIFESP EPM, Dept Physiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUNIBAN, Sect Neuromotor Rehabil, BR-02071013 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, ICB, Dept Anat, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP EPM, Dept Physiol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent57-67
dc.identifierhttp://dx.doi.org/10.1016/S0014-4886(02)00046-8
dc.identifier.citationExperimental Neurology. San Diego: Academic Press Inc Elsevier Science, v. 181, n. 1, p. 57-67, 2003.
dc.identifier.doi10.1016/S0014-4886(02)00046-8
dc.identifier.issn0014-4886
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27230
dc.identifier.wosWOS:000182527400007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Neurology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjecthippocampusen
dc.subjectepilepsyen
dc.subjectmossy fiber sproutingen
dc.subjectCGRPen
dc.subjectcycloheximideen
dc.subjectmossy cellsen
dc.titleSprouting of mossy fibers and the vacating of postsynaptic targets in the inner molecular layer of the dentate gyrusen
dc.typeinfo:eu-repo/semantics/article
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