Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

dc.contributor.authorSilva, T.m.a.
dc.contributor.authorAlves, L.g. [UNIFESP]
dc.contributor.authorQueiroz, K.c.s. de
dc.contributor.authorSantos, M.g.l.
dc.contributor.authorMarques, C.t.
dc.contributor.authorChavante, Suely Ferreira [UNIFESP]
dc.contributor.authorRocha, Hugo Alexandre Oliveira [UNIFESP]
dc.contributor.authorLeite, E.l.
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:31:33Z
dc.date.available2015-06-14T13:31:33Z
dc.date.issued2005-04-01
dc.description.abstractThe brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.en
dc.description.affiliationUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia
dc.description.affiliationUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Bioquímica
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Bioquímica
dc.description.sourceSciELO
dc.format.extent523-533
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2005000400005
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 4, p. 523-533, 2005.
dc.identifier.doi10.1590/S0100-879X2005000400005
dc.identifier.fileS0100-879X2005000400005.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2005000400005
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/2502
dc.identifier.wosWOS:000228962700005
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFucanen
dc.subjectAnticoagulant activityen
dc.subjectSulfated polysaccharidesen
dc.subjectBrown algaeen
dc.subjectPadina gymnosporaen
dc.titlePartial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnosporaen
dc.typeinfo:eu-repo/semantics/article
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