Heterofucans from Dictyota menstrualis have anticoagulant activity

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dc.contributor.authorAlbuquerque, Ivan Rui Lopes
dc.contributor.authorQueiroz, Karla Cristiana Souza
dc.contributor.authorAlves, Luciana Guimarães [UNIFESP]
dc.contributor.authorSantos, Elizeu Antunes dos [UNIFESP]
dc.contributor.authorLeite, Edda Lisboa [UNIFESP]
dc.contributor.authorRocha, Hugo Alexandre Oliveira [UNIFESP]
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:30:16Z
dc.date.available2015-06-14T13:30:16Z
dc.date.issued2004-02-01
dc.description.abstractFucan is a term used to denote a family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix of brown seaweed and in the egg jelly coat of sea urchins. Plant fucans have several biological activities, including anticoagulant and antithrombotic, related to the structural and chemical composition of polysaccharides. We have extracted sulfated polysaccharides from the brown seaweed Dictyota menstrualis by proteolytic digestion, followed by separation into 5 fractions by sequential acetone precipitation. Gel electrophoresis using 0.05 M 1,3-diaminopropane-acetate buffer, pH 9.0, stained with 0.1% toluidine blue, showed the presence of sulfated polysaccharides in all fractions. The chemical analyses demonstrated that all fractions are composed mainly of fucose, xylose, galactose, uronic acid, and sulfate. The anticoagulant activity of these heterofucans was determined by activated partial thromboplastin time (APTT) using citrate normal human plasma. Only the fucans F1.0v and F1.5v showed anticoagulant activity. To prolong the coagulation time to double the baseline value in the APTT, the required concentration of fucan F1.0v (20 µg/ml) was only 4.88-fold higher than that of the low molecular weight heparin Clexane® (4.1 µg/ml), whereas 80 µg/ml fucan 1.5 was needed to obtain the same effect. For both fucans this effect was abolished by desulfation. These polymers are composed of fucose, xylose, uronic acid, galactose, and sulfate at molar ratios of 1.0:0.8:0.7:0.8:0.4 and 1.0:0.3:0.4:1.5:1.3, respectively. This is the fist report indicating the presence of a heterofucan with higher anticoagulant activity from brown seaweed.en
dc.description.affiliationUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais
dc.description.affiliationUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Bioquímica Disciplina de Biologia Molecular
dc.description.affiliationUnifespUNIFESP, Depto. de Bioquímica Disciplina de Biologia Molecular
dc.description.sourceSciELO
dc.format.extent167-171
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2004000200002
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 2, p. 167-171, 2004.
dc.identifier.doi10.1590/S0100-879X2004000200002
dc.identifier.fileS0100-879X2004000200002.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2004000200002
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/1978
dc.identifier.wosWOS:000189185500002
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFucanen
dc.subjectFucoidanen
dc.subjectAnticoagulant drugsen
dc.subjectBrown algaeen
dc.subjectAntithrombotic agentsen
dc.titleHeterofucans from Dictyota menstrualis have anticoagulant activityen
dc.typeinfo:eu-repo/semantics/article
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