In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment

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dc.citation.issue2
dc.citation.volume88
dc.contributor.authorNasser Caetano, Livia de Vasconcelos [UNIFESP]
dc.contributor.authorMendes, Thiago de Oliveira
dc.contributor.authorBagatin, Edileia [UNIFESP]
dc.contributor.authorMiot, Helio Amante
dc.contributor.authorMarques Soares, Juliana Laudiceia [UNIFESP]
dc.contributor.authorEnokihara, Milvia Maria Simoes e Silva [UNIFESP]
dc.contributor.authorMartin, Airton Abrahao
dc.coverageClare
dc.date.accessioned2020-09-01T13:21:26Z
dc.date.available2020-09-01T13:21:26Z
dc.date.issued2017
dc.description.abstractBackground: In vivo confocal Raman spectroscopy is a non-invasive method to assess either the epidermis or the dermis composition. Few studies have focused on dermis collagen alterations through intrinsic aging and photoaging. Objective: This study evaluated the in vivo Raman spectra from the dermis of a photoexposed site versus a non-photoexposed region in different age groups, and evaluated the correlation between peak intensities and age, photoaging score and the amount of collagen assessed with histology and high frequency ultrasound (HFUS). Methods: Fifteen volunteers aged 28-82 years were divided into three groups according to forearm photoaging degree. In vivo Raman spectra from the dermis were collected on the dorsal forearm (chronically photoexposed skin) and on the proximal medial arm (non-photoexposed skin). Crosssectional images of the skin were obtained using a 20 MHz ultrasound unit exactly on the same sites, which were further submitted to punch biopsies for histologic study (collagen I immunohistochemistry, picrosirius red staining and Verhoeff). Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) were taken in the spectral region of 796 cm(-1) -996 cm(-1) to determine its potential to discriminate between different groups. The Spearman rank correlation coefficient of individual peak intensities and ratios with age, clinical score and the amount of collagen assessed by ultrasound and histology were calculated. Results: PCA of pairs of groups and OPLS-DA could discriminate the intrinsically from the photoaged skin and the young group from the elderly one, with important contribution of the 938 cm(-1) and 855 cm(-1) peaks intensities. The intensity of the peaks in 855 cm(-1) and/or 938 cm(-1) presented moderate correlation with age (rho = 0.579, p = 0.049) and moderate to high inverse correlation with HFUS echogenicity (rho = -0.710, p = 0.010) and collagen I immunohistochemistry (rho = -0.833, p = 0.005) in the non-photoexposed region. The I-1275/I-1450 intensities ratio presented moderate to high correlation coefficients with age (rho = -0.730, p = 0.007), photoaging score (rho = -0.594, p = 0.042), HFUS echogenicity (rho = 0.760, p < 0.001) and histology (collagen I immunohistochemistry (rho = 0.643, p = 0.024), picrosirius (rho = 0.773, p = 0.005) and Verhoeff (rho = -0.727, p = 0.011)) in the photoexposed site. Conclusion: The wavenumber region between 798 and 994 cm(-1) is useful for the analysis of dermal collagen alterations through the intrinsic aging process, while photoaging is better assessed by the I-1275/I-1450 intensities ratio. This is the first skin aging study to show a correlation between Raman peaks and the amount of collagen assessed by HFUS- and histology. (C) 2017 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Dept Dermatol, Rua Borges Lagoa 508, BR-04038001 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Vale Paraiba, UNIVAP, Inst Res & Dev, Lab Biomed Vibrat Spect,LEVB, Ave Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Dermatol & Radiotherapy, Campus Univ Rubiao Jr, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Piaui, Minist Petronio Portella, Campus Univ, BR-64049550 Teresina, PI, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Dept Dermatol, Rua Borges Lagoa 508, BR-04038001 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFUNADERSP, an organisation of the Sao Paulo regional office of the Brazilian Society of Dermatology
dc.description.sponsorshipIDFUNADERSP: 001/2013
dc.format.extent199-206
dc.identifierhttp://dx.doi.org/10.1016/j.jdermsci.2017.07.011
dc.identifier.citationJournal Of Dermatological Science. Clare, v. 88, n. 2, p. 199-206, 2017.
dc.identifier.doi10.1016/j.jdermsci.2017.07.011
dc.identifier.issn0923-1811
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58260
dc.identifier.wosWOS:000416193500006
dc.language.isoeng
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofJournal Of Dermatological Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectHigh frequency ultrasounden
dc.subjectHistologyen
dc.subjectPhotoagingen
dc.subjectRaman spectroscopyen
dc.subjectSkin agingen
dc.titleIn vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessmenten
dc.typeinfo:eu-repo/semantics/article
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