Affected and non-affected skin fibroblasts from systemic sclerosis patients share a gene expression profile deviated from the one observed in healthy individuals

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dc.contributor.authorFuzii, Hellen Thais [UNIFESP]
dc.contributor.authorYoshikawa, Gilberto Toshimitsu [UNIFESP]
dc.contributor.authorJunta, C. M.
dc.contributor.authorSandrin-Garcia, P.
dc.contributor.authorFachin, A. L.
dc.contributor.authorSakamoto-Hojo, E. T.
dc.contributor.authorDonadi, E. A.
dc.contributor.authorPassos, G. A. S.
dc.contributor.authorAndrade, Luiz Eduardo Coelho [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-06-15T16:52:42Z
dc.date.available2018-06-15T16:52:42Z
dc.date.issued2008-09-01
dc.description.abstractObjectivesTo evaluate the gene expression profile of fibroblasts from affected and non-affected skin of systemic sclerosis (SSc) patients and from controls.Materials and methodsLabeled cDNA from fibroblast cultures from forearm (affected) and axillary (non-affected) skin from six diffuse SSc patients, from three normal controls, and from MOLT-4/HEp-2/normal fibroblasts (reference pool) was probed in microarrays generated with 4193 human cDNAs from the IMAGE Consortium. Microarray images were converted into numerical data and gene expression was calculated as the ratio between fibroblast cDNA (Cy5) and reference pool cDNA (Cy3) data and analyzed by R environment/Aroma, Cluster, Tree View, and SAM softwares. Differential expression was confirmed by real time PCR for a set of selected genes.ResultsEighty-eight genes were up- and 241 genes down-regulated in SSc fibroblasts. Gene expression correlation was strong between affected and non-affected fibroblast samples from the same patient (r>0.8), moderate among fibroblasts from all patients (r=0.72) and among fibroblasts from all controls (r=0.70), and modest among fibroblasts from patients and controls (r=0.55). The differential expression was confirmed by real time PCR for all selected genes.ConclusionsFibroblasts from affected and non-affected skin of SSc patients shared a similar abnormal gene expression profile, suggesting that the widespread molecular disturbance in SSc fibroblasts is more sensitive than histological and clinical alterations. Novel molecular elements potentially involved in SSc pathogenesis were identified.en
dc.description.affiliationUniv Fed Sao Paulo, Disciplina Reumatol, Div Rheumatol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Mol Immunogenet Grp, Dept Genet, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Dent, Dept Morphol, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Disciplina Reumatol, Div Rheumatol, BR-04023062 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent866-874
dc.identifierhttp://www.clinexprheumatol.org/article.asp?a=3499
dc.identifier.citationClinical And Experimental Rheumatology. Pisa: Clinical & Exper Rheumatology, v. 26, n. 5, p. 866-874, 2008.
dc.identifier.issn0392-856X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43317
dc.identifier.wosWOS:000261248600017
dc.language.isoeng
dc.publisherClinical & Exper Rheumatology
dc.relation.ispartofClinical And Experimental Rheumatology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSystemic sclerosisen
dc.subjectgene expressionen
dc.subjectfibroblasten
dc.subjectcDNA microarraysen
dc.titleAffected and non-affected skin fibroblasts from systemic sclerosis patients share a gene expression profile deviated from the one observed in healthy individualsen
dc.typeinfo:eu-repo/semantics/article
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