Suppressive effect of low-level laser therapy on tracheal hyperresponsiveness and lung inflammation in rat subjected to intestinal ischemia and reperfusion

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dc.contributor.authorLima, Flavia Mafra de
dc.contributor.authorVitoretti, Luana
dc.contributor.authorCoelho, Fernando
dc.contributor.authorAlbertini, Regiane
dc.contributor.authorBreithaupt-Faloppa, Ana Cristina
dc.contributor.authorLima, Wothan Tavares de
dc.contributor.authorAimbire, Flavio [UNIFESP]
dc.contributor.institutionUniv Nove Julho
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Quim
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:11Z
dc.date.available2016-01-24T14:31:11Z
dc.date.issued2013-02-01
dc.description.abstractIntestinal ischemia and reperfusion (i-I/R) is an insult associated with acute respiratory distress syndrome (ARDS). It is not known if pro- and anti-inflammatory mediators in ARDS induced by i-I/R can be controlled by low-level laser therapy (LLLT). This study was designed to evaluate the effect of LLLT on tracheal cholinergic reactivity dysfunction and the release of inflammatory mediators from the lung after i-I/R. Anesthetized rats were subjected to superior mesenteric artery occlusion (45 min) and killed after clamp release and preestablished periods of intestinal reperfusion (30 min, 2 or 4 h). the LLLT (660 nm, 7.5 J/cm(2)) was carried out by irradiating the rats on the skin over the right upper bronchus for 15 and 30 min after initiating reperfusion and then euthanizing them 30 min, 2, or 4 h later. Lung edema was measured by the Evans blue extravasation technique, and pulmonary neutrophils were determined by myeloperoxidase (MPO) activity. Pulmonary tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10), intercellular adhesion molecule-1 (ICAM-1), and isoform of NO synthase (iNOS) mRNA expression were analyzed by real-time PCR. TNF-alpha, IL-10, and iNOS proteins in the lung were measured by the enzyme-linked immunoassay technique. LLLT (660 nm, 7.5 J/cm(2)) restored the tracheal hyperresponsiveness and hyporesponsiveness in all the periods after intestinal reperfusion. Although LLLT reduced edema and MPO activity, it did not do so in all the postreperfusion periods. It was also observed with the ICAM-1 expression. in addition to reducing both TNF-alpha and iNOS, LLLT increased IL-10 in the lungs of animals subjected to i-I/R. the results indicate that LLLT can control the lung's inflammatory response and the airway reactivity dysfunction by simultaneously reducing both TNF-alpha and iNOS.en
dc.description.affiliationUniv Nove Julho, Dept Rehabil Sci, UNINOVE, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Farmacol, Lab Fisiopatol Expt, BR-05508900 São Paulo, Brazil
dc.description.affiliationInst Quim, Dept Bioquim, Lab Radicais Livres, BR-0550800 São Paulo, Brazil
dc.description.affiliationHosp Clin FMUSP, Lab Cirurgia Cardiovasc & Fisiopatol Circulacao L, BR-01246093 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Sci & Technol, UNIFESP, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Sci & Technol, UNIFESP, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2008/08048-4
dc.description.sponsorshipIDFAPESP: 2008/08838-5
dc.format.extent551-564
dc.identifierhttp://dx.doi.org/10.1007/s10103-012-1088-1
dc.identifier.citationLasers in Medical Science. London: Springer London Ltd, v. 28, n. 2, p. 551-564, 2013.
dc.identifier.doi10.1007/s10103-012-1088-1
dc.identifier.issn0268-8921
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35914
dc.identifier.wosWOS:000315579400027
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLasers in Medical Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectGut ischemiaen
dc.subjectAcute lung inflammationen
dc.subjectAirway smooth muscleen
dc.subjectAdhesion moleculeen
dc.subjectCytokinesen
dc.subjectPhototherapyen
dc.titleSuppressive effect of low-level laser therapy on tracheal hyperresponsiveness and lung inflammation in rat subjected to intestinal ischemia and reperfusionen
dc.typeinfo:eu-repo/semantics/article
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