Hippocampal NMDA receptor blockade impairs CREB phosphorylation in amygdala after contextual fear conditioning

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dc.contributor.authorOliveira Coelho, Cesar Augusto de [UNIFESP]
dc.contributor.authorFerreira, Tatiana Lima
dc.contributor.authorKramer Soares, Juliana Carlota [UNIFESP]
dc.contributor.authorMenezes Oliveira, Maria Gabriela [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:31:56Z
dc.date.available2016-01-24T14:31:56Z
dc.date.issued2013-07-01
dc.description.abstractIn contextual fear conditioning (CFC), hippocampus is thought to process environmental stimuli into a configural representation of the context and send it to amygdala nuclei, which current evidences point to be the site of CS-US association and fear memory storage. If it is true, hippocampus should influence learning-induced plasticity in the amygdala nuclei after CFC acquisition. To test this, we infused wistar rats with saline or AP5, a NMDA receptor antagonist, in the dorsal hippocampus just before a CFC session, in which they were conditioned to a single shock, exposed to the context with no shocks or received an immediate shock. the rats were perfused, their brains harvested and immunohistochemically stained for cAMP element binding protein (CREB) phosphorylation ratio (pCREB/CREB) in lateral (LA), basal (B) and central (CeA) amygdala nuclei. CFC showed a learning-specific increase in pCREB ratio in B and CeA, in conditioned-saline rats compared to context and immediate shocked ones. Further, conditioned rats that received AP5 showed a decrease in pCREB ratio in LA, B and CeA. Our results support the current ideas that the role of hippocampus in contextual fear conditioning occurs by sending contextual information to amygdala to serve as conditioned stimulus. (c) 2013 Wiley Periodicals, Inc.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psicobiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psicobiol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2010/02509-0
dc.description.sponsorshipIDFAPESP: 2010/16295-1
dc.description.sponsorshipIDCNPq: 308652/2011-8
dc.format.extent545-551
dc.identifierhttp://dx.doi.org/10.1002/hipo.22118
dc.identifier.citationHippocampus. Hoboken: Wiley-Blackwell, v. 23, n. 7, p. 545-551, 2013.
dc.identifier.doi10.1002/hipo.22118
dc.identifier.issn1050-9631
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36468
dc.identifier.wosWOS:000320776600001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofHippocampus
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectcontextual fearen
dc.subjectplasticity inductionen
dc.subjectBLAen
dc.subjectAP5en
dc.subjectemotional memoryen
dc.titleHippocampal NMDA receptor blockade impairs CREB phosphorylation in amygdala after contextual fear conditioningen
dc.typeinfo:eu-repo/semantics/article
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