The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi

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dc.contributor.authorReis, Flavia Coelho Garcia dos
dc.contributor.authorJudice, Wagner AS
dc.contributor.authorJuliano, Maria Aparecida [UNIFESP]
dc.contributor.authorJuliano, Luiz [UNIFESP]
dc.contributor.authorScharfstein, Julio
dc.contributor.authorLima, Ana Paula CD
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:12Z
dc.date.available2016-01-24T12:41:12Z
dc.date.issued2006-06-01
dc.description.abstractPapain-like cysteine proteases are important for the survival of the flagellated protozoa Trypanosoma cruzi, the causative agent of Chagas' Disease. the lysosomal cysteine protease designated as cruzipain or cruzain, is the archetype of a multigene family of related isoforms. We investigated the substrate specificity of the cruzipain 2 isoform using internally quenched fluorogenic substrates. We found that cruzipain 2 and cruzain differ substantially regarding the specificity in the S-2, S-1(') and S-2(') pockets. Our study indicates that cruzipain 2 has a more restricted specificity than cruzain, suggesting that these isoforms might act on distinct natural substrates.en
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21949900 Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent215-220
dc.identifierhttp://dx.doi.org/10.1111/j.1574-6968.2006.00267.x
dc.identifier.citationFems Microbiology Letters. Oxford: Blackwell Publishing, v. 259, n. 2, p. 215-220, 2006.
dc.identifier.doi10.1111/j.1574-6968.2006.00267.x
dc.identifier.issn0378-1097
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28927
dc.identifier.wosWOS:000237739500008
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofFems Microbiology Letters
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcysteine proteaseen
dc.subjecttrypanosomeen
dc.subjectChagas Diseaseen
dc.subjectspecificityen
dc.subjectisoformen
dc.titleThe substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzien
dc.typeinfo:eu-repo/semantics/article
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