Diagnosis of chronic lymphoproliferative disorders by flow cytometry using four-color combinations for immunophenotyping: A proposal of the brazilian group of flow cytometry (GBCFLUX)

Página do item simplificado
dc.contributor.authorSales, M. M.
dc.contributor.authorFerreira, S. I. A. C. P.
dc.contributor.authorIkoma, M. R. V.
dc.contributor.authorSandes, A. F.
dc.contributor.authorBeltrame, M. P.
dc.contributor.authorBacal, N. S.
dc.contributor.authorSilva, M. C. A.
dc.contributor.authorMalvezzi, M.
dc.contributor.authorLorand-Metze, I. G. H.
dc.contributor.authorOrfao, A.
dc.contributor.authorYamamoto, M. [UNIFESP]
dc.date.accessioned2019-08-19T11:48:40Z
dc.date.available2019-08-19T11:48:40Z
dc.date.issued2017
dc.description.abstractBackgroundMultiparametric flow cytometry (MFC) is a powerful tool for the diagnosis of hematological malignancies and has been useful for the classification of chronic lymphoproliferative disorders (CLPD) according to the WHO criteria. Following the purposes of the Brazilian Group of Flow Cytometry (GBCFLUX), the aim of this report was to standardize the minimum requirements to achieve an accurate diagnosis in CLPDs, considering the different economic possibilities of the laboratories in our country. Most laboratories in Brazil work with 4-fluorescence flow cytometers, which is why the GBCFLUX CLPD Committee has proposed 4-color monoclonal antibody (MoAb) panels. Methods/ResultsPanels for screening and diagnosis in B, T and NK lymphoproliferative disorders were developed based on the normal differentiation pathways of these cells and the most frequent phenotypic aberrations. Important markers for prognosis and for minimal residual disease (MRD) evaluation were also included. The MoAb panels presented here were designed based on the diagnostic expertise of the participating laboratories and an extensive literature review. ConclusionThe 4-color panels presented to aid in the diagnosis of lymphoproliferative neoplasms by GBCFLUX aim to provide clinical laboratories with a systematic, step-wise, cost-effective, and reproducible approach to obtain an accurate immunophenotypic diagnosis of the most frequent of these disorders. (c) 2016 International Clinical Cytometry Societyen
dc.description.affiliationUniv São Paulo, Hosp Clin, Fac Med, São Paulo, SP, Brazil
dc.description.affiliationCtr Hematol & Hemoterapia Santa Catarina HEMOSC, Florianopolis, SC, Brazil
dc.description.affiliationHosp Amaral Carvalho Jau, Jau, SP, Brazil
dc.description.affiliationFleury Grp, Div Hematol & Flow Cytometry, São Paulo, SP, Brazil
dc.description.affiliationHosp Clin UFPR, Unidade Apoio Diagnost, Curitiba, Parana, Brazil
dc.description.affiliationHosp Albert Einstein, São Paulo, SP, Brazil
dc.description.affiliationUniv Fed Parana, Dept Clin Med, Disciplina Hematol, Curitiba, Parana, Brazil
dc.description.affiliationUniv Estadual Campinas, Campinas, SP, Brazil
dc.description.affiliationUniv Salamanca, Canc Res Ctr IBMCC, CSIC USAL, Inst Biomed Res Salamanca IBSAL,Cytometry Serv, Salamanca, Spain
dc.description.affiliationUniv Salamanca, Dept Med, Salamanca, Spain
dc.description.affiliationUniv Fed São Paulo EPM UNIFESP, Escola Paulista Med, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo EPM UNIFESP, Escola Paulista Med, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent398-410
dc.identifierhttp://dx.doi.org/10.1002/cyto.b.21396
dc.identifier.citationCytometry Part B-Clinical Cytometry. Hoboken, v. 92, n. 5, p. 398-410, 2017.
dc.identifier.doi10.1002/cyto.b.21396
dc.identifier.issn1552-4949
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51317
dc.identifier.wosWOS:000409938500010
dc.language.isoeng
dc.publisherWiley
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectchronic lymphoproliferative disordersen
dc.subjectflow cytometryen
dc.subjectGBCFLUXen
dc.subjectmonoclonal antibody panelen
dc.subjectlymphomaen
dc.subjectmultiple myelomaen
dc.titleDiagnosis of chronic lymphoproliferative disorders by flow cytometry using four-color combinations for immunophenotyping: A proposal of the brazilian group of flow cytometry (GBCFLUX)en
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos