Evaluation of Toll-like, chemokine, and integrin receptors on monocytes and neutrophils from peripheral blood of septic patients and their correlation with clinical outcomes

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dc.contributor.authorSilva, Selma Cristina da [UNIFESP]
dc.contributor.authorBaggio-Zappia, Giovana Lotici [UNIFESP]
dc.contributor.authorBrunialti, Milena Karina Coló [UNIFESP]
dc.contributor.authorAssunçao, M.s.c.
dc.contributor.authorAzevedo, Luciano Cesar Pontes [UNIFESP]
dc.contributor.authorMachado, Flávia Ribeiro [UNIFESP]
dc.contributor.authorSalomão, Reinaldo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHospital Israelita Albert Einstein Unidade de Terapia Intensiva
dc.contributor.institutionHospital Sírio Libanês Unidade de Terapia Intensiva
dc.date.accessioned2015-06-14T13:47:06Z
dc.date.available2015-06-14T13:47:06Z
dc.date.issued2014-05-02
dc.description.abstractRecognition of pathogens is performed by specific receptors in cells of the innate immune system, which may undergo modulation during the continuum of clinical manifestations of sepsis. Monocytes and neutrophils play a key role in host defense by sensing and destroying microorganisms. This study aimed to evaluate the expression of CD14 receptors on monocytes; CD66b and CXCR2 receptors on neutrophils; and TLR2, TLR4, TLR5, TLR9, and CD11b receptors on both cell types of septic patients. Seventy-seven septic patients (SP) and 40 healthy volunteers (HV) were included in the study, and blood samples were collected on day zero (D0) and after 7 days of therapy (D7). Evaluation of the cellular receptors was carried out by flow cytometry. Expression of CD14 on monocytes and of CD11b and CXCR2 on neutrophils from SP was lower than that from HV. Conversely, expression of TLR5 on monocytes and neutrophils was higher in SP compared with HV. Expression of TLR2 on the surface of neutrophils and that of TLR5 on monocytes and neutrophils of SP was lower at D7 than at D0. In addition, SP who survived showed reduced expression of TLR2 and TLR4 on the surface of neutrophils at D7 compared to D0. Expression of CXCR2 for surviving patients was higher at follow-up compared to baseline. We conclude that expression of recognition and cell signaling receptors is differentially regulated between SP and HV depending on the receptor being evaluated.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Hospital São Paulo
dc.description.affiliationHospital Israelita Albert Einstein Unidade de Terapia Intensiva
dc.description.affiliationHospital Sírio Libanês Unidade de Terapia Intensiva
dc.description.affiliationUnifespUNIFESP, EPM, Hospital São Paulo
dc.description.sourceSciELO
dc.format.extent384-393
dc.identifierhttp://dx.doi.org/10.1590/1414-431X20143190
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 47, n. 5, p. 384-393, 2014.
dc.identifier.doi10.1590/1414-431X20143190
dc.identifier.fileS0100-879X2014000500384.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2014000500384
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8379
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSepsisen
dc.subjectToll-like receptorsen
dc.subjectTLR5en
dc.subjectTLR9en
dc.subjectCXCR2en
dc.titleEvaluation of Toll-like, chemokine, and integrin receptors on monocytes and neutrophils from peripheral blood of septic patients and their correlation with clinical outcomesen
dc.typeinfo:eu-repo/semantics/article
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