Serodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude exoantigens

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dc.contributor.authorFernandes, Geisa Ferreira [UNIFESP]
dc.contributor.authorLopes-Bezerra, Leila Maria
dc.contributor.authorBernardes-Engemann, Andrea Reis
dc.contributor.authorPacheco Schubach, Tania Maria
dc.contributor.authorGalvao Dias, Maria Adelaide
dc.contributor.authorPereira, Sandro Antonio
dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.contributor.institutionFiocruz MS
dc.contributor.institutionCtr Zoonosis Prefeitura São Paulo
dc.date.accessioned2016-01-24T14:06:04Z
dc.date.available2016-01-24T14:06:04Z
dc.date.issued2011-01-27
dc.description.abstractThe main objective of this study is to standardize an ELISA for the diagnosis of feline sporotrichosis. Sporothrix schenckii is the etiological agent of human and animal sporotrichosis. Cats may act as reservoirs for S. schenckii and can transmit the infection to humans by a bite or scratch. There are few methods for the serological diagnosis of fungal diseases in animals. in this paper, an ELISA test for the diagnosis of cat sporotrichosis is proposed, which detects S. schenckii-specific antibodies in feline sera. Two different kinds of antigens were used: SsCBF, a specific molecule from S. schenckii that consists of a Con A-binding fraction derived from a peptido-rhamnomannan component of the cell wall, and a S. schenckii crude exoantigen preparation. the ELISA was developed, optimized, and evaluated using sera from 30 cats with proven sporotrichosis (by culture isolation); 22 sera from healthy feral cats from a zoonosis center were used as negative controls. SsCBF showed 90% sensitivity and 96% specificity in ELISA; while crude exoantigens demonstrated 96% sensitivity and 98% specificity. the ELISA assay described here would be a valuable screening tool for the detection of specifics. schenckii antibodies in cats with sporotrichosis. the assay is inexpensive, quick to perform, easy to interpret, and permits the diagnosis of feline sporotrichosis. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Estado Rio de Janeiro, Inst Biol IBRAG, Rio de Janeiro, Brazil
dc.description.affiliationFiocruz MS, Inst Oswaldo Cruz, Inst Pesquisa Clin Evandro Chagas, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationCtr Zoonosis Prefeitura São Paulo, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent445-449
dc.identifierhttp://dx.doi.org/10.1016/j.vetmic.2010.07.007
dc.identifier.citationVeterinary Microbiology. Amsterdam: Elsevier B.V., v. 147, n. 3-4, p. 445-449, 2011.
dc.identifier.doi10.1016/j.vetmic.2010.07.007
dc.identifier.issn0378-1135
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33363
dc.identifier.wosWOS:000287119100030
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Microbiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectSporothrix schenckiien
dc.subjectSporotrichosisen
dc.subjectELISAen
dc.subjectSsCBF antigenen
dc.subjectCrude exoantigensen
dc.subjectFelineen
dc.subjectCaten
dc.titleSerodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude exoantigensen
dc.typeinfo:eu-repo/semantics/article
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