Influence of colloid particle profile on sentinel lymph node uptake

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dc.contributor.authorFernandez Nunez, Eutimio Gustavo
dc.contributor.authorFaintuch, Bluma Linkowski
dc.contributor.authorTeodoro, Rodrigo
dc.contributor.authorWiecek, Danielle Pereira
dc.contributor.authorMartinelli, Jose Roberto
dc.contributor.authorSilva, Natanael Gomes da
dc.contributor.authorCastanheira, Claudia E.
dc.contributor.authorOliveira Filho, Renato Santos de [UNIFESP]
dc.contributor.authorPasqualini, Roberto
dc.contributor.institutionInst Energet & Nucl Res
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionCIS Bio Int
dc.date.accessioned2016-01-24T13:58:48Z
dc.date.available2016-01-24T13:58:48Z
dc.date.issued2009-10-01
dc.description.abstractIntroduction: Particle size of colloids employed for sentinel lymph node (LN) detection is not well studied. This investigation aimed to correlate particle size and distribution of different products with LN uptake.Methods: All agents (colloidal tin, dextran, phytate and colloidal rhenium sulfide) were labeled with (99m)Tc according to manufacturer's instructions. Sizing of particles was carried out oil electron micrographs using Image Tool for Windows (Version 2.0). Biodistribution studies in main excretion organs as well as in popliteal LN were Performed in male Wistar rats [30 and 90 min post injection (p.i.)]. the injected dose was 0.1 ml (37 MBq) in the footpad of the left posterior limb. Dynamic images (0-15 min p.i.) as well as static ones (30 and 90 min) were acquired in gamma camera.Results: Popliteal LN was clearly reached by all products. Nevertheless, particle size remarkably influenced node uptake. Colloidal rhenium sulfide, with the smallest diameter (5.1 x 10(-3)+/- 3.9x10(-3) mu m), permitted the best result [2.72 +/- 0.64 percent injected dose (%ID) at 90 min]. Phytate displayed small particles (<15 mu m) with favorable uptake (1.02 +/- 0.14%ID). Dextran (21.4 +/- 12.8 mu m) and colloidal tin (39.0 +/- 8.3 mu m) were less effective (0.55 +/- 0.14 and 0.06 +/- 0.03%ID respectively). Particle distribution also tended to influence results. When asymmetric, it was associated with biphasic uptake which increased over time; conversely, symmetric distribution (colloidal tin) was consistent with a constant pattern.Conclusion: the results are suggesting that particle size and symmetry may interfere with LN radiopharmaceutical uptake. (C) 2009 Elsevier Inc. All rights reserved.en
dc.description.affiliationInst Energet & Nucl Res, Radiopharm Ctr, BR-05508000 São Paulo, Brazil
dc.description.affiliationInst Energet & Nucl Res, Ctr Mat Sci & Technol, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med, BR-04020041 São Paulo, Brazil
dc.description.affiliationCIS Bio Int, Res & Dev, F-91192 Gif Sur Yvette, France
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med, BR-04020041 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent741-747
dc.identifierhttp://dx.doi.org/10.1016/j.nucmedbio.2009.04.009
dc.identifier.citationNuclear Medicine and Biology. New York: Elsevier B.V., v. 36, n. 7, p. 741-747, 2009.
dc.identifier.doi10.1016/j.nucmedbio.2009.04.009
dc.identifier.issn0969-8051
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31863
dc.identifier.wosWOS:000269852700005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNuclear Medicine and Biology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTechnetium colloidsen
dc.subjectsentinel lymph nodeen
dc.subject(99m)Tc-dextranen
dc.subject(99m)Tc-phytateen
dc.subject(99m)Tc-colloidal tinen
dc.subject(99m)Tc-colloidal rhenium sulfideen
dc.titleInfluence of colloid particle profile on sentinel lymph node uptakeen
dc.typeinfo:eu-repo/semantics/article
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